• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g SUZ12 antibody (STJ110096). Western blot was performed from the immunoprecipitate using SUZ12 antibody (STJ110096) at a dilution of 1:500.
  • Immunofluorescence analysis of C6 cells using [KO Validated] SUZ12 Rabbit polyclonal antibody (STJ110096) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and SUZ12 knockout (KO) HeLa cells, using [KO Validated] SUZ12 Rabbit polyclonal antibody (STJ110096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using [KO Validated] SUZ12 Rabbit polyclonal antibody (STJ110096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SUZ12 antibody (470-739) (STJ110096)

SKU:
STJ110096

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUZ12 (470-739) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUZ12
Gene ID: 23512
Uniprot ID: SUZ12_HUMAN
Immunogen Region: 470-739
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2).
Immunogen Sequence: CHSRFIFNYVYHPKGARIDV SINECYDGSYAGNPQDIHRQ PGFAFSRNGPVKRTPITHIL VCRPKRTKASMSEFLESEDG EVEQQRTYSSGHNRLYFHSD TCLPLRPQEMEVDSEDEKDP EWLREKTITQIEEFSDVNEG EKEVMKLWNLHVMKHGFIAD NQMNHACMLFVENYGQKIIK KNLCRNFMLHLVSMHDFNLI SIMSIDKAVTKLREMQQKLE KGESASPANEEITEEQNGT
Tissue Specificity Overexpressed in breast and colon cancer.
Post Translational Modifications Sumoylated, probably by PIAS2.
Function Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Protein Name Polycomb Protein Suz12
Chromatin Precipitated E2f Target 9 Protein
Chet 9 Protein
Joined To Jazf1 Protein
Suppressor Of Zeste 12 Protein Homolog
Database Links Reactome: R-HSA-212300
Reactome: R-HSA-2559580
Reactome: R-HSA-3214841
Reactome: R-HSA-4551638
Reactome: R-HSA-5617472
Reactome: R-HSA-8943724
Reactome: R-HSA-8953750
Reactome: R-HSA-9609690
Reactome: R-HSA-9710421
Cellular Localisation Nucleus
Localizes To Chromatin As Part Of The Prc2 Complex
Alternative Antibody Names Anti-Polycomb Protein Suz12 antibody
Anti-Chromatin Precipitated E2f Target 9 Protein antibody
Anti-Chet 9 Protein antibody
Anti-Joined To Jazf1 Protein antibody
Anti-Suppressor Of Zeste 12 Protein Homolog antibody
Anti-SUZ12 antibody
Anti-CHET9 antibody
Anti-JJAZ1 antibody
Anti-KIAA0160 antibody

Information sourced from Uniprot.org

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