• Western blot analysis of extracts of mouse skeletal muscle, using SULT2A1 antibody (STJ115998) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 50s.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using SULT2A1 rabbit polyclonal antibody (STJ115998) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using SULT2A1 rabbit polyclonal antibody (STJ115998) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using SULT2A1 rabbit polyclonal antibody (STJ115998) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using SULT2A1 rabbit polyclonal antibody (STJ115998) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using SULT2A1 rabbit polyclonal antibody (STJ115998) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SULT2A1 antibody (1-145) (STJ115998)

SKU:
STJ115998

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SULT2A1 (1-145) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SULT2A1
Gene ID: 6822
Uniprot ID: ST2A1_HUMAN
Immunogen Region: 1-145
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human SULT2A1 (NP_003158.2).
Immunogen Sequence: MSDDFLWFEGIAFPTMGFRS ETLRKVRDEFVIRDEDVIIL TYPKSGTNWLAEILCLMHSK GDAKWIQSVPIWERSPWVES EIGYTALSETESPRLFSSHL PIQLFPKSFFSSKAKVIYLM RNPRDVLVSGYFFWKNMKFI KKPKS
Tissue Specificity Liver, adrenal and at lower level in the kidney. Is present in human fetus in higher level in the adrenal than the liver and the kidney.
Post Translational Modifications The N-terminus is blocked.
Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Mediates the sulfation of a wide range of steroids and sterols, including pregnenolone, androsterone, DHEA, bile acids, cholesterol and as well many xenobiotics that contain alcohol and phenol functional groups. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Plays an important role in maintening steroid and lipid homeostasis. Plays a key role in bile acid metabolism. In addition, catalyzes the metabolic activation of potent carcinogenic polycyclic arylmethanols.
Protein Name Sulfotransferase 2a1
St2a1
Bile Salt Sulfotransferase
Dehydroepiandrosterone Sulfotransferase
Dhea-St
Dhea-St8
Hydroxysteroid Sulfotransferase
Hst
St2
Sult2a3
Database Links Reactome: R-HSA-156584
Reactome: R-HSA-1989781
Reactome: R-HSA-9753281
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Sulfotransferase 2a1 antibody
Anti-St2a1 antibody
Anti-Bile Salt Sulfotransferase antibody
Anti-Dehydroepiandrosterone Sulfotransferase antibody
Anti-Dhea-St antibody
Anti-Dhea-St8 antibody
Anti-Hydroxysteroid Sulfotransferase antibody
Anti-Hst antibody
Anti-St2 antibody
Anti-Sult2a3 antibody
Anti-SULT2A1 antibody
Anti-HST antibody
Anti-STD antibody

Information sourced from Uniprot.org

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