• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g STRAP antibody (STJ11104299). Western blot was performed from the immunoprecipitate using STRAP antibody (STJ11104299) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using STRAP Rabbit polyclonal antibody (STJ11104299) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using STRAP Rabbit polyclonal antibody (STJ11104299) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of H9C2 cells using STRAP Rabbit polyclonal antibody (STJ11104299) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and STRAP knockout (KO) HeLa cells, using STRAP Rabbit polyclonal antibody (STJ11104299) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-STRAP antibody (1-350) (STJ11104299)

SKU:
STJ11104299

Current Stock:
Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Serine-Threonine Kinase Receptor-Associated Protein (1-350) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STRAP
Gene ID: 11171
Uniprot ID: STRAP_HUMAN
Immunogen Region: 1-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human STRAP (NP_009109.3).
Immunogen Sequence: MAMRQTPLTCSGHTRPVVDL AFSGITPYGYFLISACKDGK PMLRQGDTGDWIGTFLGHKG AVWGATLNKDATKAATAAAD FTAKVWDAVSGDELMTLAHK HIVKTVDFTQDSNYLLTGGQ DKLLRIYDLNKPEAEPKEIS GHTSGIKKALWCSEDKQILS ADDKTVRLWDHATMTEVKSL NFNMSVSSMEYIPEGEILVI TYGRSIAFHSAVSLDPIKSF EAPATINSASLHPEKEFLV
Function The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. STRAP plays a role in the cellular distribution of the SMN complex. Negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein.
Protein Name Serine-Threonine Kinase Receptor-Associated Protein
Map Activator With Wd Repeats
Unr-Interacting Protein
Wd-40 Repeat Protein Pt-Wd
Database Links Reactome: R-HSA-2173788
Cellular Localisation Cytoplasm
Nucleus
Localized Predominantly In The Cytoplasm But Also Found In The Nucleus
Alternative Antibody Names Anti-Serine-Threonine Kinase Receptor-Associated Protein antibody
Anti-Map Activator With Wd Repeats antibody
Anti-Unr-Interacting Protein antibody
Anti-Wd-40 Repeat Protein Pt-Wd antibody
Anti-STRAP antibody
Anti-MAWD antibody
Anti-UNRIP antibody

Information sourced from Uniprot.org

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