• Immunofluorescence analysis of U2OS cells using SSRP1 antibody (STJ28496).
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using SSRP1 antibody (STJ28496) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse brain, using SSRP1 Rabbit polyclonal antibody antibody (STJ28496) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SSRP1 antibody (1-300) (STJ28496)

SKU:
STJ28496

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SSRP1 (1-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SSRP1
Gene ID: 6749
Uniprot ID: SSRP1_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SSRP1 (NP_003137.1).
Immunogen Sequence: MAETLEFNDVYQEVKGSMND GRLRLSRQGIIFKNSKTGKV DNIQAGELTEGIWRRVALGH GLKLLTKNGHVYKYDGFRES EFEKLSDFFKTHYRLELMEK DLCVKGWNWGTVKFGGQLLS FDIGDQPVFEIPLSNVSQCT TGKNEVTLEFHQNDDAEVSL MEVRFYVPPTQEDGVDPVEA FAQNVLSKADVIQATGDAIC IFRELQCLTPRGRYDIRIYP TFLHLHGKTFDYKIPYTTV
Post Translational Modifications Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity. Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein. Sumoylated.
Function Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
Protein Name Fact Complex Subunit Ssrp1
Chromatin-Specific Transcription Elongation Factor 80 Kda Subunit
Facilitates Chromatin Transcription Complex 80 Kda Subunit
Fact 80 Kda Subunit
Factp80
Facilitates Chromatin Transcription Complex Subunit Ssrp1
Recombination Signal Sequence Recognition Protein 1
Structure-Specific Recognition Protein 1
Hssrp1
T160
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-167152
Reactome: R-HSA-167200
Reactome: R-HSA-167238
Reactome: R-HSA-167243
Reactome: R-HSA-167246
Reactome: R-HSA-167287
Reactome: R-HSA-167290
Reactome: R-HSA-674695
Reactome: R-HSA-6796648
Reactome: R-HSA-6804756
Reactome: R-HSA-75955
Cellular Localisation Nucleus
Nucleolus
Chromosome
Colocalizes With Rna Polymerase Ii On Chromatin
Recruited To Actively Transcribed Loci
Alternative Antibody Names Anti-Fact Complex Subunit Ssrp1 antibody
Anti-Chromatin-Specific Transcription Elongation Factor 80 Kda Subunit antibody
Anti-Facilitates Chromatin Transcription Complex 80 Kda Subunit antibody
Anti-Fact 80 Kda Subunit antibody
Anti-Factp80 antibody
Anti-Facilitates Chromatin Transcription Complex Subunit Ssrp1 antibody
Anti-Recombination Signal Sequence Recognition Protein 1 antibody
Anti-Structure-Specific Recognition Protein 1 antibody
Anti-Hssrp1 antibody
Anti-T160 antibody
Anti-SSRP1 antibody
Anti-FACT80 antibody

Information sourced from Uniprot.org

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