• Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells using 3 Mu g SSB antibody (STJ25701). Western blot was performed from the immunoprecipitate using SSB antibody (STJ25701) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using SSB Rabbit polyclonal antibody (STJ25701) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SSB in paraffin-embedded human thyroid cancer using SSB Rabbit polyclonal antibody (STJ25701) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SSB in paraffin-embedded human lung cancer using SSB Rabbit polyclonal antibody (STJ25701) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SSB in paraffin-embedded human esophageal cancer using SSB Rabbit polyclonal antibody (STJ25701) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SSB in paraffin-embedded human breast cancer using SSB Rabbit polyclonal antibody (STJ25701) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SSB Rabbit polyclonal antibody (STJ25701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SSB antibody (166-408) (STJ25701)

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STJ25701

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SSB (166-408) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:500
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SSB
Gene ID: 6741
Uniprot ID: LA_HUMAN
Immunogen Region: 166-408
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 166-408 of human SSB (NP_003133.1).
Immunogen Sequence: KFVETPGQKYKETDLLILFK DDYFAKKNEERKQNKVEAKL RAKQEQEAKQKLEEDAEMKS LEEKIGCLLKFSGDLDDQTC REDLHILFSNHGEIKWIDFV RGAKEGIILFKEKAKEALGK AKDANNGNLQLRNKEVTWEV LEGEVEKEALKKIIEDQQES LNKWKSKGRRFKGKGKGNKA AQPGSGKGKVQFQGKKTKFA SDDEHDEHDENGATGPVKRA REETDKEEPASKQQKTENG
Post Translational Modifications Phosphorylated. The phosphorylation sites are at the C-terminal part of the protein. The N-terminus is blocked.
Function Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation. In case of Coxsackievirus B3 infection, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation.
Protein Name Lupus La Protein
La Autoantigen
La Ribonucleoprotein
Sjoegren Syndrome Type B Antigen
Ss-B
Database Links Reactome: R-HSA-73980
Reactome: R-HSA-749476
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Lupus La Protein antibody
Anti-La Autoantigen antibody
Anti-La Ribonucleoprotein antibody
Anti-Sjoegren Syndrome Type B Antigen antibody
Anti-Ss-B antibody
Anti-SSB antibody

Information sourced from Uniprot.org

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