• Immunofluorescence analysis of U2OS cells using SRSF9 antibody (STJ114412) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using SRSF9 antibody (STJ114412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SRSF9 antibody (1-221) (STJ114412)

SKU:
STJ114412

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SFRS9 (1-221) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRSF9
Gene ID: 8683
Uniprot ID: SRSF9_HUMAN
Immunogen Region: 1-221
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human SFRS9 (NP_003760.1).
Immunogen Sequence: MSGWADERGGEGDGRIYVGN LPTDVREKDLEDLFYKYGRI REIELKNRHGLVPFAFVRFE DPRDAEDAIYGRNGYDYGQC RLRVEFPRTYGGRGGWPRGG RNGPPTRRSDFRVLVSGLPP SGSWQDLKDHMREAGDVCYA DVQKDGVGMVEYLRKEDMEY ALRKLDDTKFRSHEGETSYI RVYPERSTSYGYSRSRSGSR GRDSPYQSRGSPHYFSPFRP Y
Tissue Specificity Expressed at high levels in the heart, kidney, pancreas and placenta, and at lower levels in the brain, liver, lung and skeletal muscle.
Post Translational Modifications Extensively phosphorylated on serine residues in the RS domain.
Function Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10.
Protein Name Serine/Arginine-Rich Splicing Factor 9
Pre-Mrna-Splicing Factor Srp30c
Splicing Factor - Arginine/Serine-Rich 9
Database Links Reactome: R-HSA-159236
Reactome: R-HSA-72163
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Cellular Localisation Nucleus
Cellular Stresses Such As Heat Shock May Induce Localization To Discrete Nuclear Bodies Termed Sam68 Nuclear Bodies (Snbs)
Hap Bodies
Or Stress Bodies
Numerous Splicing Factors Including Srsf1/Sfrs1/Sf2
Srsf7/Sfrs7
Safb And Khdrbs1/Sam68 Accumulate At These Structures
Which May Participate In The Post-Transcriptional Regulation Of Mrnas In Stressed Cells
Alternative Antibody Names Anti-Serine/Arginine-Rich Splicing Factor 9 antibody
Anti-Pre-Mrna-Splicing Factor Srp30c antibody
Anti-Splicing Factor - Arginine/Serine-Rich 9 antibody
Anti-SRSF9 antibody
Anti-SFRS9 antibody
Anti-SRP30C antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance