Anti-SRSF3 antibody (52-65 aa) [R01-6G2] (STJA0012690)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human SFRS3
General Information
| Short Description | Rabbit monoclonal anti-SRSF3 (52-65 aa) for use in WB and IHC-P in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R01-6G2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100 |
| Formulation | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | SRSF3 |
| Gene ID | 6428 |
| Uniprot ID | SRSF3_HUMAN |
| Immunogen | A synthetic peptide of human SFRS3 |
| Immunogen Region | 52-65 aa |
Additional Info
| Post Translational Modifications | Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain. |
| Function | Splicing factor, which binds the consensus motif 5'-CACUAUCACUACC-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons. Also functions as an adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway).enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. |
| Protein Name | Serine/Arginine-Rich Splicing Factor 3Pre-Mrna-Splicing Factor Srp20Splicing Factor - Arginine/Serine-Rich 3 |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-72203Reactome: R-HSA-73856 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasmRecruited To Nuclear Speckles Following Interaction With Ythdc1 |
| Alternative Antibody Names | Anti-Serine/Arginine-Rich Splicing Factor 3 antibodyAnti-Pre-Mrna-Splicing Factor Srp20 antibodyAnti-Splicing Factor - Arginine/Serine-Rich 3 antibodyAnti-SRSF3 antibodyAnti-SFRS3 antibodyAnti-SRP20 antibody |
Information sourced from Uniprot.org