• Western blot analysis of lysates from HeLa and Jurkat cells, using SFRS3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of MCF7 cells, using SFRS3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of 293T lysis using SRp20 antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of KB lysis using SRp20 antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of Jurkat cells using SRp20 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of various cells using SRp20 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-SRSF3 antibody (111-160 aa) (STJ95782)

SKU:
STJ95782

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Serine/arginine-rich splicing factor 3 (111-160 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRSF3
Gene ID: 6428
Uniprot ID: SRSF3_HUMAN
Immunogen Region: 111-160 aa
Specificity: SRp20 Polyclonal Antibody detects endogenous levels of SRp20 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SFRS3 at the amino acid range 111-160
Function Splicing factor that specifically promotes exon-inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. Also functions as export adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway).enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. RNA-binding is semi-sequence specific.
Protein Name Serine/Arginine-Rich Splicing Factor 3
Pre-Mrna-Splicing Factor Srp20
Splicing Factor - Arginine/Serine-Rich 3
Database Links Reactome: R-HSA-159236
Reactome: R-HSA-72163
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Cellular Localisation Nucleus
Nucleus Speckle
Cytoplasm
Recruited To Nuclear Speckles Following Interaction With Ythdc1
Alternative Antibody Names Anti-Serine/Arginine-Rich Splicing Factor 3 antibody
Anti-Pre-Mrna-Splicing Factor Srp20 antibody
Anti-Splicing Factor - Arginine/Serine-Rich 3 antibody
Anti-SRSF3 antibody
Anti-SFRS3 antibody
Anti-SRP20 antibody

Information sourced from Uniprot.org

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