• Immunohistochemistry analysis of paraffin-embedded mouse brain using SRP19 antibody (STJ28835) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human vermiform appendix using SRP19 antibody (STJ28835) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using SRP19 antibody (STJ28835) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SRP19 antibody (STJ28835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SRP19 antibody (1-144) (STJ28835)

SKU:
STJ28835

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SRP19 (1-144) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRP19
Gene ID: 6728
Uniprot ID: SRP19_HUMAN
Immunogen Region: 1-144
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SRP19 (NP_003126.1).
Immunogen Sequence: MACAAARSPADQDRFICIYP AYLNNKKTIAEGRRIPISKA VENPTATEIQDVCSAVGLNV FLEKNKMYSREWNRDVQYRG RVRVQLKQEDGSLCLVQFPS RKSVMLYAAEMIPKLKTRTQ KTGGADQSLQQGEGSKKGKG KKKK
Function Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). Binds directly to 7SL RNA. Mediates binding of SRP54 to the SRP complex.
Protein Name Signal Recognition Particle 19 Kda Protein
Srp19
Database Links Reactome: R-HSA-1799339
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Nucleoplasm
Although The Signal Recognition Particle Complex Acts In The Cytoplasm
It Assembles At Least In Part In The Nucleus And/Or The Nucleolus
Srp19 Nuclear Import May Be Mediated By Ipo8/Imp8 And Tpno1/Trn
Alternative Antibody Names Anti-Signal Recognition Particle 19 Kda Protein antibody
Anti-Srp19 antibody
Anti-SRP19 antibody

Information sourced from Uniprot.org

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