Anti-SPTLC2 antibody (423-562) (STJ113315)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SPTLC2 (423-562) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SPTLC2 |
| Gene ID | 9517 |
| Uniprot ID | SPTC2_HUMAN |
| Immunogen Region | 423-562 |
| Immunogen Sequence | MKCIMGQDGTSLGKECVQQL AENTRYFRRRLKEMGFIIYG NEDSPVVPLMLYMPAKIGAF GREMLKRNIGVVVVGFPATP IIESRARFCLSAAHTKEILD TALKEIDEVGDLLQLKYSRH RLVPLLDRPFDETTYEETED |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 423-562 of human SPTLC2 (NP_004854.1). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is composed of SPTLC1, SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer consisting of SPTLC1 and SPTLC2/SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Crucial for adipogenesis. |
| Protein Name | Serine Palmitoyltransferase 2Long Chain Base Biosynthesis Protein 2Lcb 2Long Chain Base Biosynthesis Protein 2aLcb2aSerine-Palmitoyl-Coa Transferase 2Spt 2 |
| Database Links | Reactome: R-HSA-1660661 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Serine Palmitoyltransferase 2 antibodyAnti-Long Chain Base Biosynthesis Protein 2 antibodyAnti-Lcb 2 antibodyAnti-Long Chain Base Biosynthesis Protein 2a antibodyAnti-Lcb2a antibodyAnti-Serine-Palmitoyl-Coa Transferase 2 antibodyAnti-Spt 2 antibodyAnti-SPTLC2 antibodyAnti-KIAA0526 antibodyAnti-LCB2 antibody |
Information sourced from Uniprot.org