• Western blot analysis of extracts of C6 cells, using Sprouty 2 antibody (STJ11103625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human appendix using Sprouty 2 Rabbit monoclonal antibody (STJ11103625) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat pancreas using Sprouty 2 rabbit monoclonal antibody (STJ11103625) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat pancreas using Sprouty 2 Rabbit monoclonal antibody (STJ11103625) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human appendix using Sprouty 2 rabbit monoclonal antibody (STJ11103625) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of C6 cells, using Sprouty 2 antibody (STJ11103625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Sprouty 2 antibody (216-315) [S5MR] (STJ11103625)

SKU:
STJ11103625

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Sprouty 2 (216-315) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPRY2
Gene ID: 10253
Uniprot ID: SPY2_HUMAN
Immunogen Region: 216-315
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 216-315 of human Sprouty 2 (O43597).
Immunogen Sequence: GTCVCCVKGLFYHCSNDDED NCADNPCSCSQSHCCTRWSA MGVMSLFLPCLWCYLPAKGC LKLCQGCYDRVNRPGCRCKN SNTVCCKVPTVPPRNFEKPT
Post Translational Modifications Cleaved at Pro-144 by the prolyl endopeptidase FAP (seprase) activity (in vitro).
Function Antagonist of fibroblast growth factor (FGF) pathways via inhibition of FGF-mediated phosphorylation of ERK1/2. Thereby acts as an antagonist of FGF-induced retinal lens fiber differentiation, may inhibit limb bud outgrowth and may negatively modulate respiratory organogenesis. Inhibits TGFB-induced epithelial-to-mesenchymal transition in retinal lens epithelial cells. Inhibits CBL/C-CBL-mediated EGFR ubiquitination.
Protein Name Protein Sprouty Homolog 2
Spry-2
Database Links Reactome: R-HSA-1295596
Reactome: R-HSA-182971
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Ruffle Membrane
Associated With Microtubules In Unstimulated Cells But Is Translocated To The Membrane Ruffles In Cells Stimulated With Egf (Epidermal Growth Factor)
Alternative Antibody Names Anti-Protein Sprouty Homolog 2 antibody
Anti-Spry-2 antibody
Anti-SPRY2 antibody

Information sourced from Uniprot.org

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