| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SPRED1 (120-320) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SPRED1 |
| Gene ID: |
161742 |
| Uniprot ID: |
SPRE1_HUMAN |
| Immunogen Region: |
120-320 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 120-320 of human SPRED1 (NP_689807.1). |
| Immunogen Sequence: |
IEDISQGCPESKNEAEGADD LQANEEDSSSSLVKDHLFQQ ETVVTSEPYRSSNIRPSPFE DLNARRVYMQSQANQITFGQ PGLDIQSRSMEYVQRQISKE CGSLKSQNRVPLKSIRHVSF QDEDEIVRINPRDILIRRYA DYRHPDMWKNDLERDDADSS IQFSKPDSKKSDYLYSCGDE TKLSSPKDSVVFKTQPSSLK I |
| Tissue Specificity | Weakly expressed in embryonic cell line HEK293. |
| Post Translational Modifications | Palmitoylated by ZDHHC17/HIP14. Phosphorylated on tyrosine. Ubiquitinated. |
| Function | Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow. Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2. Attenuates actin stress fiber formation via inhibition of TESK1-mediated phosphorylation of cofilin. Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells. |
| Protein Name | Sprouty-Related - Evh1 Domain-Containing Protein 1Spred-1Hspred1 |
| Database Links | Reactome: R-HSA-5658442Reactome: R-HSA-5658623Reactome: R-HSA-6802953 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinMembraneCaveolaNucleusLocalized In Cholesterol-Rich Membrane Raft/Caveola Fractions |
| Alternative Antibody Names | Anti-Sprouty-Related - Evh1 Domain-Containing Protein 1 antibodyAnti-Spred-1 antibodyAnti-Hspred1 antibodyAnti-SPRED1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of PC-12 cells using [KO Validated] KEAP1 Rabbit polyclonal antibody (STJ11104499) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/177143/368561/STJ11104499_1__57159.1713134043.jpg?c=1 "Immunofluorescence analysis of PC-12 cells using [KO Validated] KEAP1 Rabbit polyclonal antibody (STJ11104499) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")