• Immunofluorescence analysis of HeLa cells using SPINT1 antibody (STJ28494). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using SPINT1 antibody (STJ28494) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-SPINT1 antibody (234-513) (STJ28494)

SKU:
STJ28494

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPINT1 (234-513) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPINT1
Gene ID: 6692
Uniprot ID: SPIT1_HUMAN
Immunogen Region: 234-513
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 234-513 of human SPINT1 (NP_003701.1).
Immunogen Sequence: ANVTVTVLSTKQTEDYCLAS NKVGRCRGSFPRWYYDPTEQ ICKSFVYGGCLGNKNNYLRE EECILACRGVQGPSMERRHP VCSGTCQPTQFRCSNGCCID SFLECDDTPNCPDASDEAAC EKYTSGFDELQRIHFPSDKG HCVDLPDTGLCKESIPRWYY NPFSEHCARFTYGGCYGNKN NFEEEQQCLESCRGISKKDV FGLRREIPIPSTGSVEMAVA VFLVICIVVVVAILGYCFF
Function Inhibitor of HGFAC. Inhibits serine protease activity of ST14/matriptase in vitro. Inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain.
Protein Name Kunitz-Type Protease Inhibitor 1
Hepatocyte Growth Factor Activator Inhibitor Type 1
Hai-1
Database Links Reactome: R-HSA-6806942
Reactome: R-HSA-8852405
Cellular Localisation Secreted
Cytoplasm
Cell Membrane
Alternative Antibody Names Anti-Kunitz-Type Protease Inhibitor 1 antibody
Anti-Hepatocyte Growth Factor Activator Inhibitor Type 1 antibody
Anti-Hai-1 antibody
Anti-SPINT1 antibody
Anti-HAI1 antibody
Anti-UNQ223 antibody
Anti-PRO256 antibody

Information sourced from Uniprot.org

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