• Immunofluorescence analysis of U-2 OS cells using [KO Validated] SOD2 Rabbit polyclonal antibody (STJ25654) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] SOD2 Rabbit polyclonal antibody (STJ25654) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using SOD2 Rabbit polyclonal antibody (STJ25654) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from wild type (WT) and SOD2 knockout (KO) 293T cells, using SOD2 Rabbit polyclonal antibody (STJ25654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using SOD2 antibody (STJ25654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SOD2 antibody (25-222) (STJ25654)

SKU:
STJ25654

$174.83 - $867.38
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOD2
Gene ID: 6648
Uniprot ID: SODM_HUMAN
Immunogen Region: 25-222
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 25-222 of human SOD2 (NP_000627.2).
Immunogen Sequence: KHSLPDLPYDYGALEPHINA QIMQLHHSKHHAAYVNNLNV TEEKYQEALAKGDVTAQIAL QPALKFNGGGHINHSIFWTN LSPNGGGEPKGELLEAIKRD FGSFDKFKEKLTAASVGVQG SGWGWLGFNKERGHLQIAAC PNQDPLQGTTGLIPLLGIDV WEHAYYLQYKNVRPDYLKAI WNVINWENVTERYMACKK
Post Translational Modifications Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting. Polyubiquitinated.leading to proteasomal degradation. Deubiquitinated by USP36 which increases protein stability.
Function Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Protein Name Superoxide Dismutase Mn - Mitochondrial
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-3299685
Reactome: R-HSA-8862803
Reactome: R-HSA-8950505
Reactome: R-HSA-9615017
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Superoxide Dismutase Mn - Mitochondrial antibody
Anti-SOD2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance