![Immunofluorescence analysis ofย U-2 OS cells usingย [KO Validated] SOD2 Rabbit polyclonal antibody (STJ25654) atย aย dilution ofย 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100562/387218/STJ25654_1__98061.1713156451.jpg?c=1 "Immunofluorescence analysis ofย U-2 OS cells usingย [KO Validated] SOD2 Rabbit polyclonal antibody (STJ25654) atย aย dilution ofย 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis ofย NIH/3T3 cells usingย [KO Validated] SOD2 Rabbit polyclonal antibody (STJ25654) atย aย dilution ofย 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100562/387219/STJ25654_2__55620.1713156452.jpg?c=1 "Immunofluorescence analysis ofย NIH/3T3 cells usingย [KO Validated] SOD2 Rabbit polyclonal antibody (STJ25654) atย aย dilution ofย 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis ofย U-2 OS cells usingย [KO Validated] SOD2 Rabbit polyclonal antibody (STJ25654) atย aย dilution ofย 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/100562/387218/STJ25654_1__98061.1713156451.jpg?c=1 "Immunofluorescence analysis ofย U-2 OS cells usingย [KO Validated] SOD2 Rabbit polyclonal antibody (STJ25654) atย aย dilution ofย 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-SOD2 antibody (25-222) (STJ25654)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SOD2 (25-222) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SOD2 |
| Gene ID | 6648 |
| Uniprot ID | SODM_HUMAN |
| Immunogen Region | 25-222 |
| Immunogen Sequence | KHSLPDLPYDYGALEPHINA QIMQLHHSKHHAAYVNNLNV TEEKYQEALAKGDVTAQIAL QPALKFNGGGHINHSIFWTN LSPNGGGEPKGELLEAIKRD FGSFDKFKEKLTAASVGVQG SGWGWLGFNKERGHLQIAAC PNQDPLQGTTGLIPLLGIDV WEHAYYLQYKNVRPDYLKAI WNVINWENVTERYMACKK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 25-222 of human SOD2 (NP_000627.2). |
Additional Info
| Post Translational Modifications | Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting. Polyubiquitinated.leading to proteasomal degradation. Deubiquitinated by USP36 which increases protein stability. |
| Function | Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. |
| Protein Name | Superoxide Dismutase Mn - Mitochondrial |
| Database Links | Reactome: R-HSA-2151201Reactome: R-HSA-3299685Reactome: R-HSA-8862803Reactome: R-HSA-8950505Reactome: R-HSA-9615017Reactome: R-HSA-9841251 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Superoxide Dismutase Mn - Mitochondrial antibodyAnti-SOD2 antibody |
Information sourced from Uniprot.org