• Immunohistochemistry analysis of SNRNP70 in paraffin-embedded mouse kidney using SNRNP70 Rabbit polyclonal antibody (STJ27859) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SNRNP70 in paraffin-embedded human cervix cancer using SNRNP70 Rabbit polyclonal antibody (STJ27859) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SNRNP70 in paraffin-embedded rat testis using SNRNP70 Rabbit polyclonal antibody (STJ27859) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SNRNP70 Rabbit polyclonal antibody (STJ27859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SNRNP70 antibody (150-250) (STJ27859)

SKU:
STJ27859

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SNRNP70 (150-250) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNRNP70
Gene ID: 6625
Uniprot ID: RU17_HUMAN
Immunogen Region: 150-250
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SNRNP70 (NP_003080.2).
Immunogen Sequence: EYEHERDMHSAYKHADGKKI DGRRVLVDVERGRTVKGWRP RRLGGGLGGTRRGGADVNIR HSGRDDTSRYDERPGPSPLP HRDRDRDRERERRERSRERD K
Post Translational Modifications The N-terminus is blocked. Extensively phosphorylated on serine residues in the C-terminal region.
Function Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. Isoform 3: Truncated isoforms that lack the RRM domain cannot bind U1-snRNA. Isoform 4: Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.
Protein Name U1 Small Nuclear Ribonucleoprotein 70 Kda
U1 Snrnp 70 Kda
U1-70k
Snrnp70
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus Speckle
Nucleus
Nucleoplasm
Colocalizes With Scnm1 And Luc7l2 In Nuclear Speckles
Alternative Antibody Names Anti-U1 Small Nuclear Ribonucleoprotein 70 Kda antibody
Anti-U1 Snrnp 70 Kda antibody
Anti-U1-70k antibody
Anti-Snrnp70 antibody
Anti-SNRNP70 antibody
Anti-RNPU1Z antibody
Anti-RPU1 antibody
Anti-SNRP70 antibody
Anti-U1AP1 antibody

Information sourced from Uniprot.org

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