Anti-SMYD2 antibody (1-270) (STJ28557)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SMYD2 (1-270) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:10-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SMYD2 |
| Gene ID | 56950 |
| Uniprot ID | SMYD2_HUMAN |
| Immunogen Region | 1-270 |
| Immunogen Sequence | MRAEGLGGLERFCSPGKGRG LRALQPFQVGDLLFSCPAYA YVLTVNERGNHCEYCFTRKE GLSKCGRCKQAFYCNVECQK EDWPMHKLECSPMVVFGENW NPSETVRLTARILAKQKIHP ERTPSEKLLAVKEFESHLDK LDNEKKDLIQSDIAALHHFY SKHLGFPDNDSLVVLFAQVN CNGFTIEDEELSHLGSAIFP DVALMNHSCCPNVIVTYKGT LAEVRAVQEIKPGEEVFTS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SMYD2 (NP_064582.2). |
Additional Info
| Function | Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 'Lys-4' (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'. |
| Protein Name | N-Lysine Methyltransferase Smyd2Hskm-BHistone Methyltransferase Smyd2Lysine N-Methyltransferase 3cSet And Mynd Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-3214841Reactome: R-HSA-6804760 |
| Cellular Localisation | CytoplasmCytosolNucleus |
| Alternative Antibody Names | Anti-N-Lysine Methyltransferase Smyd2 antibodyAnti-Hskm-B antibodyAnti-Histone Methyltransferase Smyd2 antibodyAnti-Lysine N-Methyltransferase 3c antibodyAnti-Set And Mynd Domain-Containing Protein 2 antibodyAnti-SMYD2 antibodyAnti-KMT3C antibody |
Information sourced from Uniprot.org