Anti-SMCHD1 antibody (1756-2005) (STJ29294)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SMCHD1 (1756-2005) antibody for use in WB, IF, ICC and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SMCHD1 |
| Gene ID | 23347 |
| Uniprot ID | SMHD1_HUMAN |
| Immunogen Region | 1756-2005 |
| Immunogen Sequence | TTDAARRIYDETQGRQQVLP LDSIYKKTLPDWKRSLPHFR NGKLYFKPIGDPVFARDLLT FPDNVEHCETVFGMLLGDTI ILDNLDAANHYRKEVVKITH CPTLLTRDGDRIRSNGKFGG LQNKAPPMDKLRGMVFGAPV PKQCLILGEQIDLLQQYRSA VCKLDSVNKDLNSQLEYLRT PDMRKKKQELDEHEKNLKLI EEKLGMTPIRKCNDSLRHSP KVETTDCPVPPKRMRREAT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1756-2005 of human SMCHD1 (NP_056110.2). |
Additional Info
| Post Translational Modifications | Sumoylated with SUMO1. |
| Function | Non-canonical member of the structural maintenance of chromosomes (SMC) protein family that plays a key role in epigenetic silencing by regulating chromatin architecture. Promotes heterochromatin formation in both autosomes and chromosome X, probably by mediating the merge of chromatin compartments. Plays a key role in chromosome X inactivation in females by promoting the spreading of heterochromatin. Recruited to inactivated chromosome X by Xist RNA and acts by mediating the merge of chromatin compartments: promotes random chromatin interactions that span the boundaries of existing structures, leading to create a compartment-less architecture typical of inactivated chromosome X. Required to facilitate Xist RNA spreading. Also required for silencing of a subset of clustered autosomal loci in somatic cells, such as the DUX4 locus. Has ATPase activity.may participate in structural manipulation of chromatin in an ATP-dependent manner as part of its role in gene expression regulation. Also plays a role in DNA repair: localizes to sites of DNA double-strand breaks in response to DNA damage to promote the repair of DNA double-strand breaks. Acts by promoting non-homologous end joining (NHEJ) and inhibiting homologous recombination (HR) repair. |
| Protein Name | Structural Maintenance Of Chromosomes Flexible Hinge Domain-Containing Protein 1Smc Hinge Domain-Containing Protein 1 |
| Database Links | |
| Cellular Localisation | ChromosomeRecruited To Inactivated Chromosome X In Females By Xist RnaLocalizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs) |
| Alternative Antibody Names | Anti-Structural Maintenance Of Chromosomes Flexible Hinge Domain-Containing Protein 1 antibodyAnti-Smc Hinge Domain-Containing Protein 1 antibodyAnti-SMCHD1 antibodyAnti-KIAA0650 antibody |
Information sourced from Uniprot.org