• Immunofluorescence analysis of MCF-7 cells using SMCHD1 antibody (STJ29294). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using SMCHD1 antibody (STJ29294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SMCHD1 antibody (1756-2005) (STJ29294)

SKU:
STJ29294

€140.63 - €653.13
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SMCHD1 (1756-2005) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMCHD1
Gene ID: 23347
Uniprot ID: SMHD1_HUMAN
Immunogen Region: 1756-2005
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1756-2005 of human SMCHD1 (NP_056110.2).
Immunogen Sequence: TTDAARRIYDETQGRQQVLP LDSIYKKTLPDWKRSLPHFR NGKLYFKPIGDPVFARDLLT FPDNVEHCETVFGMLLGDTI ILDNLDAANHYRKEVVKITH CPTLLTRDGDRIRSNGKFGG LQNKAPPMDKLRGMVFGAPV PKQCLILGEQIDLLQQYRSA VCKLDSVNKDLNSQLEYLRT PDMRKKKQELDEHEKNLKLI EEKLGMTPIRKCNDSLRHSP KVETTDCPVPPKRMRREAT
Post Translational Modifications Sumoylated with SUMO1.
Function Non-canonical member of the structural maintenance of chromosomes (SMC) protein family that plays a key role in epigenetic silencing by regulating chromatin architecture. Promotes heterochromatin formation in both autosomes and chromosome X, probably by mediating the merge of chromatin compartments. Plays a key role in chromosome X inactivation in females by promoting the spreading of heterochromatin. Recruited to inactivated chromosome X by Xist RNA and acts by mediating the merge of chromatin compartments: promotes random chromatin interactions that span the boundaries of existing structures, leading to create a compartment-less architecture typical of inactivated chromosome X. Required to facilitate Xist RNA spreading. Also required for silencing of a subset of clustered autosomal loci in somatic cells, such as the DUX4 locus. Has ATPase activity.may participate in structural manipulation of chromatin in an ATP-dependent manner as part of its role in gene expression regulation. Also plays a role in DNA repair: localizes to sites of DNA double-strand breaks in response to DNA damage to promote the repair of DNA double-strand breaks. Acts by promoting non-homologous end joining (NHEJ) and inhibiting homologous recombination (HR) repair.
Protein Name Structural Maintenance Of Chromosomes Flexible Hinge Domain-Containing Protein 1
Smc Hinge Domain-Containing Protein 1
Cellular Localisation Chromosome
Recruited To Inactivated Chromosome X In Females By Xist Rna
Localizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs)
Alternative Antibody Names Anti-Structural Maintenance Of Chromosomes Flexible Hinge Domain-Containing Protein 1 antibody
Anti-Smc Hinge Domain-Containing Protein 1 antibody
Anti-SMCHD1 antibody
Anti-KIAA0650 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance