Anti-SMARCAD1 antibody (1-300) (STJ28413)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ28413
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General Information

Short DescriptionRabbit polyclonal SMARCAD1 (1-300) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSMARCAD1
Gene ID56916
Uniprot IDSMRCD_HUMAN
Immunogen Region1-300
Immunogen SequenceMNLFNLDRFRFEKRNKIEEA PEATPQPSQPGPSSPISLSA EEENAEGEVSRANTPDSDIT EKTEDSSVPETPDNERKASI SYFKNQRGIQYIDLSSDSED VVSPNCSNTVQEKTFNKDTV IIVSEPSEDEESQGLPTMAR RNDDISELEDLSELEDLKDA KLQTLKELFPQRSDNDLLKL IESTSTMDGAIAAALLMFGD AGGGPRKRKLSSSSEPYEED EFNDDQSIKKTRLDHGEES
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMARCAD1 (NP_001121901.1).

Additional Info

Tissue Specificity Isoform 1 is expressed ubiquitously. Isoform 3 is expressed mainly in skin and esophagus. Expressed in fibroblasts and keratinocytes (at protein level).
Function DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing.
Protein Name Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Containing Dead/H Box 1
Smarcad1
Atp-Dependent Helicase 1
Hhel1
Database Links
Cellular Localisation Nucleus
Chromosome
Colocalizes With Pcna At Replication Forks During S Phase
Recruited To Double-Strand Breaks (Dsbs) Sites Of Dna Damage
Alternative Antibody Names Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Containing Dead/H Box 1 antibody
Anti-Smarcad1 antibody
Anti-Atp-Dependent Helicase 1 antibody
Anti-Hhel1 antibody
Anti-SMARCAD1 antibody
Anti-KIAA1122 antibody

Information sourced from Uniprot.org

Citations

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