Western blot analysis of various lysates using SMAD2/SMAD3 Rabbit pAb (STJ11101471) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-SMAD2/SMAD3 antibody (150-270) (STJ11101471)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11101471
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General Information

Short DescriptionRabbit polyclonal SMAD2/SMAD3 (150-270) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSMAD3
SMAD2
Gene ID4088
4087
Uniprot IDSMAD3_HUMAN
SMAD2_HUMAN
Immunogen Region150-270
Immunogen SequenceEYAFNLKKDEVCVNPYHYQR VETPVLPPVLVPRHTEILTE LPPLDDYTHSIPENTNFPAG IEPQSNYIPETPPPGYISED GETSDQQLNQSMDTGSPAEL SPTTLSPVNHSLDLQPVTYS E
SpecificityA synthetic peptide corresponding to a sequence within amino acids 150-270 of human SMAD2/SMAD3SMAD3 (NP_005893.1).

Additional Info

Tissue Specificity P84022 - NA
Q15796 - Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
Alternative Antibody Names Anti-Mothers Against Decapentaplegic Homolog 3 antibody
Anti-Mad Homolog 3 antibody
Anti-Mad3 antibody
Anti-Mothers Against Dpp Homolog 3 antibody
Anti-Hmad-3 antibody
Anti-Jv15-2 antibody
Anti-Smad Family Member 3 antibody
Anti-Smad 3 antibody
Anti-Smad3 antibody
Anti-Hsmad3 antibody
Anti-SMAD3 antibody
Anti-MADH3 antibody
Anti-Mothers Against Decapentaplegic Homolog 2 antibody
Anti-Mad Homolog 2 antibody
Anti-Mothers Against Dpp Homolog 2 antibody
Anti-Jv18-1 antibody
Anti-Mad-Related Protein 2 antibody
Anti-Hmad-2 antibody
Anti-Smad Family Member 2 antibody
Anti-Smad 2 antibody
Anti-Smad2 antibody
Anti-Hsmad2 antibody
Anti-SMAD2 antibody
Anti-MADH2 antibody
Anti-MADR2 antibody

Information sourced from Uniprot.org

Citations

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