Anti-SLC5A7 antibody (280-350 aa) (STJ13102213)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 280-350 of mouse SLC5A7 conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-SLC5A7 (280-350 aa) for use in IHC and WB in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1: 200 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Slc5a7 |
| Gene ID | 63993 |
| Uniprot ID | SC5A7_MOUSE |
| Immunogen | A synthetic peptide from aa region 280-350 of mouse SLC5A7 conjugated to blue carrier protein was used as the antigen. |
| Immunogen Region | 280-350 aa |
| Specificity | Specific for SLC5A7. |
Additional Info
| Post Translational Modifications | Phosphorylated by PKC and dephosphorylated by PP1/PP2A. |
| Function | High-affinity Na(+)-coupled choline transmembrane symporter. Functions as an electrogenic, voltage-dependent transporter with variable charge/choline stoichiometry. Choline uptake and choline-induced current is also Cl(-)-dependent where Cl(-) is likely a regulatory ion rather than cotransported ion. Plays a critical role in acetylcholine (ACh) synthesis by taking up the substrate choline from the synaptic cleft into the presynaptic nerve terminals after neurotransmitter release. SLC5A7/CHT1-mediated choline high-affinity transport in cholinergic neurons is the rate-limiting step for production of ACh, thereby facilitating communication by subsequent action potentials. Localized predominantly in presynaptic terminal intracellular organelles, and translocated to the plasma membrane in active form in response to neuronal activity. |
| Protein Name | High Affinity Choline Transporter 1Hemicholinium-3-Sensitive Choline TransporterChtSolute Carrier Family 5 Member 7 |
| Database Links | Reactome: R-MMU-264642Reactome: -MMU-425366 |
| Cellular Localisation | Presynaptic Cell MembraneMulti-Pass Membrane ProteinCell ProjectionAxonEarly Endosome MembraneCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneLocalized To Motor Neuron Axons |
| Alternative Antibody Names | Anti-High Affinity Choline Transporter 1 antibodyAnti-Hemicholinium-3-Sensitive Choline Transporter antibodyAnti-Cht antibodyAnti-Solute Carrier Family 5 Member 7 antibodyAnti-Slc5a7 antibodyAnti-Cht1 antibody |
Information sourced from Uniprot.org