• Western blot analysis of extracts of various cell lines, using antibody (STJ11103529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SLC3A2/CD98hc antibody (350-450) [S9MR] (STJ11103529)

SKU:
STJ11103529

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SLC3A2/CD98hc (350-450) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC3A2
Gene ID: 6520
Uniprot ID: 4F2_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human SLC3A2/CD98hc (P08195).
Immunogen Sequence: IENLKDASSFLAEWQNITKG FSEDRLLIAGTNSSDLQQIL SLLESNKDLLLTSSYLSDSG STGEHTKSLVTQYLNATGNR WCSWSLSQARLLTSFLPAQL L
Tissue Specificity Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1.
Post Translational Modifications N-glycosylated.N-glycosylation is crucial for trafficking and stability of SLC3A2 to the plasma membrane. Phosphorylation on Ser-406.Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions.
Function Acts as a chaperone that facilitates biogenesis and trafficking of functional transporters heterodimers to the plasma membrane. Forms heterodimer with SLC7 family transporters (SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10 and SLC7A11), a group of amino-acid antiporters. Heterodimers function as amino acids exchangers, the specificity of the substrate depending on the SLC7A subunit. Heterodimers SLC3A2/SLC7A6 or SLC3A2/SLC7A7 mediate the uptake of dibasic amino acids. Heterodimer SLC3A2/SLC7A11 functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane. SLC3A2/SLC7A10 translocates small neutral L- and D-amino acids across the plasma membrane. SLC3A2/SLC75 or SLC3A2/SLC7A8 translocates neutral amino acids with broad specificity, thyroid hormones and L-DOPA. SLC3A2 is essential for plasma membrane localization, stability, and the transport activity of SLC7A5 and SLC7A8. When associated with LAPTM4B, the heterodimer SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation. Modulates integrin-related signaling and is essential for integrin-dependent cell spreading, migration and tumor progression. (Microbial infection) In case of hepatitis C virus/HCV infection, the complex formed by SLC3A2 and SLC7A5/LAT1 plays a role in HCV propagation by facilitating viral entry into host cell and increasing L-leucine uptake-mediated mTORC1 signaling activation, thereby contributing to HCV-mediated pathogenesis. (Microbial infection) Acts as a receptor for malaria parasite Plasmodium vivax (Thai isolate) in immature red blood cells.
Protein Name Amino Acid Transporter Heavy Chain Slc3a2
4f2 Cell-Surface Antigen Heavy Chain
4f2hc
4f2 Heavy Chain Antigen
Lymphocyte Activation Antigen 4f2 Large Subunit
Solute Carrier Family 3 Member 2
Cd Antigen Cd98
Database Links Reactome: R-HSA-210991
Reactome: R-HSA-352230
Reactome: R-HSA-5660862
Reactome: R-HSA-71240
Cellular Localisation Apical Cell Membrane
Cell Membrane
Single-Pass Type Ii Membrane Protein
Cell Junction
Lysosome Membrane
Melanosome
Basolateral Cell Membrane
Localized At The Plasma Membrane When Associated With Slc7a5/Lat1 Or Slc7a8/Lat2
Localized To The Apical Membrane Of Placental Syncytiotrophoblastic Cells
Recruited To Lysosomes By Laptm4b
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Located Selectively At Cell-Cell Adhesion Sites
Colocalized With Slc7a8/Lat2 At The Basolateral Membrane Of Kidney Proximal Tubules And Small Intestine Epithelia
Expressed In Both Luminal And Abluminal Membranes Of Brain Capillary Endothelial Cells
Alternative Antibody Names Anti-Amino Acid Transporter Heavy Chain Slc3a2 antibody
Anti-4f2 Cell-Surface Antigen Heavy Chain antibody
Anti-4f2hc antibody
Anti-4f2 Heavy Chain Antigen antibody
Anti-Lymphocyte Activation Antigen 4f2 Large Subunit antibody
Anti-Solute Carrier Family 3 Member 2 antibody
Anti-Cd Antigen Cd98 antibody
Anti-SLC3A2 antibody
Anti-MDU1 antibody

Information sourced from Uniprot.org

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