• Western blot analysis of various lysates using SLC22A7 Rabbit polyclonal antibody (STJ117331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SLC22A7 antibody (50-142) (STJ117331)

SKU:
STJ117331

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC22A7 (50-142) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC22A7
Gene ID: 10864
Uniprot ID: S22A7_HUMAN
Immunogen Region: 50-142
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-142 of human SLC22A7 (NP_696961.2).
Immunogen Sequence: ALPGAPANFSHQDVWLEAHL PREPDGTLSSCLRFAYPQAL PNTTLGEERQSRGELEDEPA TVPCSQGWEYDHSEFSSTIA TESQWDLVCEQKG
Tissue Specificity Mainly expressed in liver and kidney. In kidney, expressed in proximal tubular cells. Also expressed in pancreas, small intestine, spinal cord, lung, brain and heart. Expressed in fetal liver.
Function Isoform 2: Functions as a Na(+)-independent bidirectional multispecific transporter. Contributes to the renal and hepatic elimination of endogenous organic compounds from the systemic circulation into the urine and bile, respectively. Capable of transporting a wide range of purine and pyrimidine nucleobases, nucleosides and nucleotides, with cGMP, 2'deoxyguanosine and GMP being the preferred substrates. Functions as a pH- and chloride-independent cGMP bidirectional facilitative transporter that can regulate both intracellular and extracellular levels of cGMP and may be involved in cGMP signaling pathways. Mediates orotate/glutamate bidirectional exchange and most likely display a physiological role in hepatic release of glutamate into the blood. Involved in renal secretion and possible reabsorption of creatinine. Able to uptake prostaglandin E2 (PGE2) and may contribute to PGE2 renal excretion (Probable). Also transports alpha-ketoglutarate and urate. Apart from the orotate/glutamate exchange, the counterions for the uptake of other SLC22A7/OAT2 substrates remain to be identified. Isoform 1: Non functional transporter. Isoform 3: Involved in the uptake of prostaglandin F2-alpha (PGF2-alpha).
Protein Name Solute Carrier Family 22 Member 7
Novel Liver Transporter
Nlt
Organic Anion Transporter 2
Hoat2
Database Links Reactome: R-HSA-561048
Reactome: R-HSA-9749641
Cellular Localisation Isoform 2: Basolateral Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Cell Membrane
Localized To The Basolateral Side Of The Proximal Tubules
Apical Side Of The Renal Tubule
Isoform 1: Cytoplasm
Cytosol
Alternative Antibody Names Anti-Solute Carrier Family 22 Member 7 antibody
Anti-Novel Liver Transporter antibody
Anti-Nlt antibody
Anti-Organic Anion Transporter 2 antibody
Anti-Hoat2 antibody
Anti-SLC22A7 antibody
Anti-NLT antibody
Anti-OAT2 antibody

Information sourced from Uniprot.org

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