• Western blot analysis of various lysates using SIX5 Rabbit polyclonal antibody (STJ118405) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SIX5 antibody (1-170) (STJ118405)

SKU:
STJ118405

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SIX5 (1-170) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIX5
Gene ID: 147912
Uniprot ID: SIX5_HUMAN
Immunogen Region: 1-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human SIX5 (NP_787071.2).
Immunogen Sequence: MATLPAEPSAGPAAGGEAVA AAAATEEEEEEARQLLQTLQ AAEGEAAAAAGAGAGAAAAG AEGPGSPGVPGSPPEAASEP PTGLRFSPEQVACVCEALLQ AGHAGRLSRFLGALPPAERL RGSDPVLRARALVAFQRGEY AELYRLLESRPFPAAHHAFL QDLYLRARYH
Tissue Specificity Expressed in adult but not in fetal eyes. Found in corneal epithelium and endothelium, lens epithelium, ciliary body epithelia, cellular layers of the retina and the sclera.
Function Transcription factor that is thought to be involved in regulation of organogenesis. May be involved in determination and maintenance of retina formation. Binds a 5'-GGTGTCAG-3' motif present in the ARE regulatory element of ATP1A1. Binds a 5'-TCAAGAGTTNC-3' motif present in the MEF3 element in the myogenin promoter, and in the IGFBP5 promoter. Thought to be regulated by association with Dach and Eya proteins, and seems to be coactivated by EYA1, EYA2 and EYA3.
Protein Name Homeobox Protein Six5
Dm Locus-Associated Homeodomain Protein
Sine Oculis Homeobox Homolog 5
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Homeobox Protein Six5 antibody
Anti-Dm Locus-Associated Homeodomain Protein antibody
Anti-Sine Oculis Homeobox Homolog 5 antibody
Anti-SIX5 antibody
Anti-DMAHP antibody

Information sourced from Uniprot.org

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