• Immunohistochemistry analysis of JMJD6 in paraffin-embedded human lung using JMJD6 Rabbit polyclonal antibody (STJ11104574) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse liver using JMJD6 Rabbit polyclonal antibody (STJ11104574) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:90s.

Anti-JMJD6 antibody (1-170) (STJ11104574)

SKU:
STJ11104574

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Bifunctional Arginine Demethylase And Lysyl-Hydroxylase Jmjd6 (1-170) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JMJD6
Gene ID: 23210
Uniprot ID: JMJD6_HUMAN
Immunogen Region: 1-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human JMJD6 (NP_055982.2).
Immunogen Sequence: MNHKSKKRIREAKRSARPEL KDSLDWTRHNYYESFSLSPA AVADNVERADALQLSVEEFV ERYERPYKPVVLLNAQEGWS AQEKWTLERLKRKYRNQKFK CGEDNDGYSVKMKMKYYIEY MESTRDDSPLYIFDSSYGEH PKRRKLLEDYKVPKFFTDDL FQYAGEKRRP
Tissue Specificity Highly expressed in the heart, skeletal muscle and kidney. Expressed at moderate or low level in brain, placenta, lung, liver, pancreas, spleen, thymus, prostate, testis and ovary. Up-regulated in many patients with chronic pancreatitis. Expressed in nursing thymic epithelial cells.
Post Translational Modifications Hydroxylates its own N-terminus.hydroxylation is required for homooligomerization.
Function Dioxygenase that can both act as a arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Regulates RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. Hydroxylates its own N-terminus, which is required for homooligomerization. Plays a role in the regulation of nucleolar liquid-liquid phase separation (LLPS) by post-translationally modifying LIAT1 at its lysine-rich domain which inhibits LIAT1 nucleolar targeting. In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which preferentially demethylates asymmetric dimethylation. Demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), including mono-, symmetric di- and asymmetric dimethylated forms, thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. In collaboration with BRD4, interacts with the positive transcription elongation factor b (P-TEFb) complex in its active form to regulate polymerase II promoter-proximal pause release for transcriptional activation of a large cohort of genes. On distal enhancers, so called anti-pause enhancers, demethylates both histone H4R3me2 and the methyl cap of 7SKsnRNA leading to the dismissal of the 7SKsnRNA:HEXIM1 inhibitor complex. After removal of repressive marks, the complex BRD4:JMJD6 attract and retain the P-TEFb complex on chromatin, leading to its activation, promoter-proximal polymerase II pause release, and transcriptional activation. Demethylates other arginine methylated-proteins such as ESR1. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.
Protein Name Bifunctional Arginine Demethylase And Lysyl-Hydroxylase Jmjd6
Histone Arginine Demethylase Jmjd6
Jmjc Domain-Containing Protein 6
Jumonji Domain-Containing Protein 6
Lysyl-Hydroxylase Jmjd6
Peptide-Lysine 5-Dioxygenase Jmjd6
Phosphatidylserine Receptor
Protein Ptdsr
Database Links Reactome: R-HSA-3214842
Cellular Localisation Nucleus
Nucleoplasm
Nucleolus
Cytoplasm
Mainly Found Throughout The Nucleoplasm Outside Of Regions Containing Heterochromatic Dna
With Some Localization In Nucleolus
During Mitosis
Excluded From The Nucleus And Reappears In The Telophase Of The Cell Cycle
Alternative Antibody Names Anti-Bifunctional Arginine Demethylase And Lysyl-Hydroxylase Jmjd6 antibody
Anti-Histone Arginine Demethylase Jmjd6 antibody
Anti-Jmjc Domain-Containing Protein 6 antibody
Anti-Jumonji Domain-Containing Protein 6 antibody
Anti-Lysyl-Hydroxylase Jmjd6 antibody
Anti-Peptide-Lysine 5-Dioxygenase Jmjd6 antibody
Anti-Phosphatidylserine Receptor antibody
Anti-Protein Ptdsr antibody
Anti-JMJD6 antibody
Anti-KIAA0585 antibody
Anti-PSR antibody
Anti-PTDSR antibody

Information sourced from Uniprot.org

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