Anti-PARL antibody (1-170) (STJ110531)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PARL (1-170) antibody for use in WB, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PARL |
| Gene ID | 55486 |
| Uniprot ID | PARL_HUMAN |
| Immunogen Region | 1-170 |
| Immunogen Sequence | MAWRGWAQRGWGCGQAWGAS VGGRSCEELTAVLTPPQLLG RRFNFFIQQKCGFRKAPRKV EPRRSDPGTSGEAYKRSALI PPVEETVFYPSPYPIRSLIK PLFFTVGFTGCAFGSAAIWQ YESLKSRVQSYFDGIKADWL DSIRPQKEGDFRKEINKWWN NLSDGQRTVT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PARL (NP_001032728.1). |
Additional Info
| Post Translational Modifications | P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner. The cleavage is inhibited when residues Ser-65, Thr-69 and Ser-70 are all phosphorylated. |
| Function | Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptotic signals. Required for the maturation of PINK1 into its 52kDa mature form after its cleavage by mitochondrial-processing peptidase (MPP). Promotes cleavage of serine/threonine-protein phosphatase PGAM5 in damaged mitochondria in response to loss of mitochondrial membrane potential. Mediates differential cleavage of PINK1 and PGAM5 depending on the health status of mitochondria, disassociating from PINK1 and associating with PGAM5 in response to mitochondrial membrane potential loss. Required for processing of CLPB into a form with higher protein disaggregase activity by removing an autoinhibitory N-terminal peptide. Promotes processing of DIABLO/SMAC in the mitochondrion which is required for DIABLO apoptotic activity. Also required for cleavage of STARD7 and TTC19. Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain. |
| Protein Name | Presenilin-Associated Rhomboid-Like Protein - MitochondrialMitochondrial Intramembrane Cleaving Protease Parl Cleaved Into - P-BetaPbeta |
| Database Links | Reactome: R-HSA-8949664 |
| Cellular Localisation | Mitochondrion Inner MembraneMulti-Pass Membrane ProteinP-Beta: NucleusTranslocated Into The Nucleus By An Unknown Mechanism |
| Alternative Antibody Names | Anti-Presenilin-Associated Rhomboid-Like Protein - Mitochondrial antibodyAnti-Mitochondrial Intramembrane Cleaving Protease Parl Cleaved Into - P-Beta antibodyAnti-Pbeta antibodyAnti-PARL antibodyAnti-PSARL antibodyAnti-PRO2207 antibody |
Information sourced from Uniprot.org