• Western blot analysis of extracts of various cell lines, using PARL antibody (STJ110531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using PARL antibody (STJ110531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PARL antibody (1-170) (STJ110531)

SKU:
STJ110531

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PARL (1-170) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PARL
Gene ID: 55486
Uniprot ID: PARL_HUMAN
Immunogen Region: 1-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PARL (NP_001032728.1).
Immunogen Sequence: MAWRGWAQRGWGCGQAWGAS VGGRSCEELTAVLTPPQLLG RRFNFFIQQKCGFRKAPRKV EPRRSDPGTSGEAYKRSALI PPVEETVFYPSPYPIRSLIK PLFFTVGFTGCAFGSAAIWQ YESLKSRVQSYFDGIKADWL DSIRPQKEGDFRKEINKWWN NLSDGQRTVT
Post Translational Modifications P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner. The cleavage is inhibited when residues Ser-65, Thr-69 and Ser-70 are all phosphorylated.
Function Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptotic signals. Required for the maturation of PINK1 into its 52kDa mature form after its cleavage by mitochondrial-processing peptidase (MPP). Promotes cleavage of serine/threonine-protein phosphatase PGAM5 in damaged mitochondria in response to loss of mitochondrial membrane potential. Mediates differential cleavage of PINK1 and PGAM5 depending on the health status of mitochondria, disassociating from PINK1 and associating with PGAM5 in response to mitochondrial membrane potential loss. Required for processing of CLPB into a form with higher protein disaggregase activity by removing an autoinhibitory N-terminal peptide. Promotes processing of DIABLO/SMAC in the mitochondrion which is required for DIABLO apoptotic activity. Also required for cleavage of STARD7 and TTC19. Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain.
Protein Name Presenilin-Associated Rhomboid-Like Protein - Mitochondrial
Mitochondrial Intramembrane Cleaving Protease Parl Cleaved Into - P-Beta
Pbeta
Database Links Reactome: R-HSA-8949664
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
P-Beta: Nucleus
Translocated Into The Nucleus By An Unknown Mechanism
Alternative Antibody Names Anti-Presenilin-Associated Rhomboid-Like Protein - Mitochondrial antibody
Anti-Mitochondrial Intramembrane Cleaving Protease Parl Cleaved Into - P-Beta antibody
Anti-Pbeta antibody
Anti-PARL antibody
Anti-PSARL antibody
Anti-PRO2207 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance