| Host: | Goat |
| Applications: | Pep-ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-SIT1 (Internal) is suitable for use in ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:2000.WB: Preliminary experiments in Human Lymph Node, Spleen, Tonsil and Thymus lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate a |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | SIT1 |
| Gene ID: | 27240 |
| Uniprot ID: | SIT1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_055265.1 |
| Immunogen Sequence: | HLSQWTRGRSRSH |
| Post Translational Modifications | Phosphorylated on tyrosines by LCK, FYN or ZAP70 upon TCR activation.which leads to the recruitment of PTPN11, GRB2 and CSK. |
| Function | Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells. |
| Protein Name | Signaling Threshold-Regulating Transmembrane Adapter 1Shp2-Interacting Transmembrane Adapter ProteinSuppression-Inducing Transmembrane Adapter 1Gp30/40 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Signaling Threshold-Regulating Transmembrane Adapter 1 antibodyAnti-Shp2-Interacting Transmembrane Adapter Protein antibodyAnti-Suppression-Inducing Transmembrane Adapter 1 antibodyAnti-Gp30/40 antibodyAnti-SIT1 antibodyAnti-SIT antibody |
Information sourced from Uniprot.org