Anti-SHARPIN antibody (STJA0006734)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human SHARPIN
General Information
| Short Description | Rabbit polyclonal anti-Sharpin for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SHARPIN |
| Gene ID | 81858 |
| Uniprot ID | SHRPN_HUMAN |
| Immunogen | Synthesized peptide derived from human SHARPIN |
| Specificity | This antibody detects endogenous levels of SHARPIN at Human, Mouse, Rat |
Additional Info
| Function | Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria. LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin. The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. |
| Protein Name | SharpinShank-Associated Rh Domain-Interacting ProteinShank-Interacting Protein-Like 1Hsipl1 |
| Database Links | Reactome: R-HSA-5357786Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-6794361 |
| Cellular Localisation | CytoplasmCytosolSynapseEnriched At Synaptic Sites In Mature Neurons Where It Colocalizes With Shank1 |
| Alternative Antibody Names | Anti-Sharpin antibodyAnti-Shank-Associated Rh Domain-Interacting Protein antibodyAnti-Shank-Interacting Protein-Like 1 antibodyAnti-Hsipl1 antibodyAnti-SHARPIN antibodyAnti-SIPL1 antibodyAnti-PSEC0216 antibody |
Information sourced from Uniprot.org