Anti-SHARPIN antibody (STJA0006734)

SKU:
STJA0006734

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Sharpin is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SHARPIN
Gene ID: 81858
Uniprot ID: SHRPN_HUMAN
Specificity: This antibody detects endogenous levels of SHARPIN at Human, Mouse, Rat
Immunogen: Synthesized peptide derived from human SHARPIN
Function Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria. LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin. The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
Protein Name Sharpin
Shank-Associated Rh Domain-Interacting Protein
Shank-Interacting Protein-Like 1
Hsipl1
Database Links Reactome: R-HSA-5357786
Reactome: R-HSA-5357905
Reactome: R-HSA-5357956
Reactome: R-HSA-6794361
Cellular Localisation Cytoplasm
Cytosol
Synapse
Enriched At Synaptic Sites In Mature Neurons Where It Colocalizes With Shank1
Alternative Antibody Names Anti-Sharpin antibody
Anti-Shank-Associated Rh Domain-Interacting Protein antibody
Anti-Shank-Interacting Protein-Like 1 antibody
Anti-Hsipl1 antibody
Anti-SHARPIN antibody
Anti-SIPL1 antibody
Anti-PSEC0216 antibody

Information sourced from Uniprot.org

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