• Immunofluorescence analysis of A-549 cells using SF3B2 antibody (STJ28148).
  • Immunohistochemistry analysis of paraffin-embedded mouse Intestine using SF3B2 antibody (STJ28148) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using SF3B2 antibody (STJ28148) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using SF3B2 antibody (STJ28148) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SF3B2 antibody (STJ28148) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SF3B2 antibody (616-895) (STJ28148)

SKU:
STJ28148

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SF3B2 (616-895) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3B2
Gene ID: 10992
Uniprot ID: SF3B2_HUMAN
Immunogen Region: 616-895
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 616-895 of human SF3B2 (NP_006833.2).
Immunogen Sequence: SLGMPVGPNAHKVPPPWLIA MQRYGPPPSYPNLKIPGLNS PIPESCSFGYHAGGWGKPPV DETGKPLYGDVFGTNAAEFQ TKTEEEEIDRTPWGELEPSD EESSEEEEEEESDEDKPDET GFITPADSGLITPGGFSSVP AGMETPELIELRKKKIEEAM DGSETPQLFTVLPEKRTATV GGAMMGSTHIYDMSTVMSRK GPAPELQGVEVALAPEELEL DPMAMTQKYEEHVREQQAQ
Post Translational Modifications Methylation at Arg-508 by PRMT9 is required for the interaction with SMN1.
Function Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
Protein Name Splicing Factor 3b Subunit 2
Pre-Mrna-Splicing Factor Sf3b 145 Kda Subunit
Sf3b145
Spliceosome-Associated Protein 145
Sap 145
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Nucleus Speckle
Alternative Antibody Names Anti-Splicing Factor 3b Subunit 2 antibody
Anti-Pre-Mrna-Splicing Factor Sf3b 145 Kda Subunit antibody
Anti-Sf3b145 antibody
Anti-Spliceosome-Associated Protein 145 antibody
Anti-Sap 145 antibody
Anti-SF3B2 antibody
Anti-SAP145 antibody

Information sourced from Uniprot.org

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