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Western blot analysis of extracts of various cell lines, using SF3B1 antibody (STJ11103676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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Confocal imaging of C2C12 cells using SF3B1 Rabbit monoclonal antibody (STJ11103676, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
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Immunohistochemistry of paraffin-embedded rat ovary using SF3B1 rabbit monoclonal antibody (STJ11103676) at dilution of 1:100 (40x lens).
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Confocal imaging of C6 cells using SF3B1 Rabbit monoclonal antibody (STJ11103676, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
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Immunohistochemistry of paraffin-embedded human appendix using SF3B1 rabbit monoclonal antibody (STJ11103676) at dilution of 1:100 (40x lens).
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Confocal imaging of HeLa cells using SF3B1 Rabbit monoclonal antibody (STJ11103676, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
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Immunohistochemistry of paraffin-embedded mouse testis using SF3B1 rabbit monoclonal antibody (STJ11103676) at dilution of 1:100 (40x lens).
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Immunohistochemistry analysis of SF3B1 in paraffin-embedded human cervix cancer tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunofluorescence analysis of C6 cells using SF3B1 rabbit monoclonal antibody (STJ11103676) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunohistochemistry analysis of SF3B1 in paraffin-embedded mouse testis tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of SF3B1 in paraffin-embedded mouse intestin tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of SF3B1 in paraffin-embedded human colon tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of SF3B1 in paraffin-embedded human colon carcinoma tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of SF3B1 in paraffin-embedded rat brain tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Western blot analysis of various lysates using SF3B1 Rabbit monoclonal antibody (STJ11103676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.