• Western blot analysis of extracts of various cell lines, using SF3B1 antibody (STJ11103676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Confocal imaging of C2C12 cells using SF3B1 Rabbit monoclonal antibody (STJ11103676, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded rat ovary using SF3B1 rabbit monoclonal antibody (STJ11103676) at dilution of 1:100 (40x lens).
  • Confocal imaging of C6 cells using SF3B1 Rabbit monoclonal antibody (STJ11103676, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded human appendix using SF3B1 rabbit monoclonal antibody (STJ11103676) at dilution of 1:100 (40x lens).
  • Confocal imaging of HeLa cells using SF3B1 Rabbit monoclonal antibody (STJ11103676, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded mouse testis using SF3B1 rabbit monoclonal antibody (STJ11103676) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of SF3B1 in paraffin-embedded human cervix cancer tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of C6 cells using SF3B1 rabbit monoclonal antibody (STJ11103676) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SF3B1 in paraffin-embedded mouse testis tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B1 in paraffin-embedded mouse intestin tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B1 in paraffin-embedded human colon tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B1 in paraffin-embedded human colon carcinoma tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B1 in paraffin-embedded rat brain tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using SF3B1 Rabbit monoclonal antibody (STJ11103676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SF3B1 antibody (1-100) [S6MR] (STJ11103676)

SKU:
STJ11103676

$197.78 - $863.33
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3B1
Gene ID: 23451
Uniprot ID: SF3B1_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SF3B1 (O75533).
Immunogen Sequence: MAKIAKTHEDIEAQIREIQG KKAALDEAQGVGLDSTGYYD QEIYGGSDSRFAGYVTSIAA TELEDDDDDYSSSTSLLGQK KPGYHAPVALLNDIPQSTEQ
Post Translational Modifications Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis. Citrullinated by PADI4.
Function Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B1 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs. Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92.
Protein Name Splicing Factor 3b Subunit 1
Pre-Mrna-Splicing Factor Sf3b 155 Kda Subunit
Sf3b155
Spliceosome-Associated Protein 155
Sap 155
Database Links Reactome: R-HSA-5250924
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Nucleus Speckle
During Mitosis
Transiently Dispersed From The Nuclear Speckles To The Cytoplasm
Alternative Antibody Names Anti-Splicing Factor 3b Subunit 1 antibody
Anti-Pre-Mrna-Splicing Factor Sf3b 155 Kda Subunit antibody
Anti-Sf3b155 antibody
Anti-Spliceosome-Associated Protein 155 antibody
Anti-Sap 155 antibody
Anti-SF3B1 antibody
Anti-SAP155 antibody

Information sourced from Uniprot.org

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