| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-SETDB2 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 90kDa band observed in Human Heart lysates (calculated MW of 81.9kDa according to NP_114121.2). Recommended concentration: 0.1-0.3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | SETDB2 |
| Gene ID: | 83852 |
| Uniprot ID: | SETB2_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_114121.2; NP_001153780.1 |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_114121.2; NP_001153780.1). |
| Immunogen Sequence: | QRRHAKTNSHSSALH |
| Function | Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. |
| Protein Name | Histone-Lysine N-Methyltransferase Setdb2Chronic Lymphocytic Leukemia Deletion Region Gene 8 ProteinLysine N-Methyltransferase 1fSet Domain Bifurcated 2 |
| Database Links | Reactome: R-HSA-3214841 |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Setdb2 antibodyAnti-Chronic Lymphocytic Leukemia Deletion Region Gene 8 Protein antibodyAnti-Lysine N-Methyltransferase 1f antibodyAnti-Set Domain Bifurcated 2 antibodyAnti-SETDB2 antibodyAnti-C13orf4 antibodyAnti-CLLD8 antibodyAnti-KMT1F antibody |
Information sourced from Uniprot.org