• Western blot analysis of extracts of various cell lines, using SETD3 antibody (STJ110374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug SETD3 antibody (STJ110374). Western blot was performed from the immunoprecipitate using SETD3 antibody (STJ110374) at a dilition of 1:1000.

Anti-SETD3 antibody (1-100) (STJ110374)

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STJ110374

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SETD3 (1-100) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SETD3
Gene ID: 84193
Uniprot ID: SETD3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SETD3 (NP_115609.2).
Immunogen Sequence: MGKKSRVKTQKSGTGATATV SPKEILNLTSELLQKCSSPA PGPGKEWEEYVQIRTLVEKI RKKQKGLSVTFDGKREDYFP DLMKWASENGASVEGFEMVN
Post Translational Modifications Phosphorylated by GSK3B, which is required for recognition by the SCF(FBXW7) complex and subsequent degradation. Ubiquitinated by the SCF(FBXW7) complex following phosphorylation by GSK3B, leading to its degradation by the proteasome.
Function Protein-histidine N-methyltransferase that specifically mediates 3-methylhistidine (tele-methylhistidine) methylation of actin at 'His-73'. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery. Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin.
Protein Name Actin-Histidine N-Methyltransferase
Protein-L-Histidine N-Tele-Methyltransferase
Set Domain-Containing Protein 3
Hsetd3
Database Links Reactome: R-HSA-3214841
Cellular Localisation Cytoplasm
Nucleus
Localizes Mainly In The Cytoplasm
Alternative Antibody Names Anti-Actin-Histidine N-Methyltransferase antibody
Anti-Protein-L-Histidine N-Tele-Methyltransferase antibody
Anti-Set Domain-Containing Protein 3 antibody
Anti-Hsetd3 antibody
Anti-SETD3 antibody
Anti-C14orf154 antibody

Information sourced from Uniprot.org

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