Anti-SERPINF1 antibody (141-240) (STJ25489)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ25489
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General Information

Short DescriptionRabbit polyclonal PEDF (141-240) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IF/ICC:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSERPINF1
Gene ID5176
Uniprot IDPEDF_HUMAN
Immunogen Region141-240
Immunogen SequenceRIVFEKKLRIKSSFVAPLEK SYGTRPRVLTGNPRLDLQEI NNWVQAQMKGKLARSTKEIP DEISILLLGVAHFKGQWVTK FDSRKTSLEDFYLDEERTVR
SpecificityA synthetic peptide corresponding to a sequence within amino acids 141-240 of human PEDF/SERPINF1 (NP_002606.3).

Additional Info

Tissue Specificity Retinal pigment epithelial cells and blood plasma.
Post Translational Modifications The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Function Neurotrophic protein.induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Protein Name Pigment Epithelium-Derived Factor
Pedf
Cell Proliferation-Inducing Gene 35 Protein
Epc-1
Serpin F1
Database Links
Cellular Localisation Secreted
Melanosome
Enriched In Stage I Melanosomes
Alternative Antibody Names Anti-Pigment Epithelium-Derived Factor antibody
Anti-Pedf antibody
Anti-Cell Proliferation-Inducing Gene 35 Protein antibody
Anti-Epc-1 antibody
Anti-Serpin F1 antibody
Anti-SERPINF1 antibody
Anti-PEDF antibody
Anti-PIG35 antibody

Information sourced from Uniprot.org

Citations

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