• Immunohistochemistry analysis of paraffin-embedded rat pancreatic islet using Secretagogin (Secretagogin (SCGN) ) Rabbit monoclonal antibody (STJ11103053) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using Secretagogin (Secretagogin (SCGN) ) Rabbit monoclonal antibody (STJ11103053) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Secretagogin (Secretagogin (SCGN) ) Rabbit monoclonal antibody (STJ11103053) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Secretagogin (Secretagogin (SCGN) ) Rabbit monoclonal antibody (STJ11103053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Secretagogin antibody (177-276) [S3MR] (STJ11103053)

SKU:
STJ11103053

€183.13 - €799.38
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SCGN (177-276) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCGN
Gene ID: 10590
Uniprot ID: SEGN_HUMAN
Immunogen Region: 177-276
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 177-276 of human Secretagogin (Secretagogin (SCGN) ) (O76038).
Immunogen Sequence: ILALQENFLLQFKMDACSTE ERKRDFEKIFAYYDVSKTGA LEGPEVDGFVKDMMELVQPS ISGVDLDKFREILLRHCDVN KDGKIQKSELALCLGLKINP
Tissue Specificity Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons.
Protein Name Secretagogin
Cellular Localisation Cytoplasm
Secreted
Cytoplasmic Vesicle
Secretory Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Predominantly Cytoplasmic
A Small Proportion Is Associated With Secretory Granules And Membrane Fractions
Detectable In Human Serum After Ischemic Neuronal Damage
Alternative Antibody Names Anti-Secretagogin antibody
Anti-SCGN antibody
Anti-SECRET antibody

Information sourced from Uniprot.org

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