• Western blot analysis of extracts of HepG2 cells, using Sec23AA rabbit monoclonal antibody (STJ11102342) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH/3T3 cells using Sec23AA Rabbit monoclonal antibody (STJ11102342) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat ovary using Sec23AA rabbit monoclonal antibody (STJ11102342) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Hep G2 cells, using Sec23AA Rabbit monoclonal antibody (STJ11102342) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded human appendix using Sec23AA rabbit monoclonal antibody (STJ11102342) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Sec23AA rabbit monoclonal antibody (STJ11102342) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using Sec23AA rabbit monoclonal antibody (STJ11102342) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SEC23A antibody (300-400) [S2MR] (STJ11102342)

SKU:
STJ11102342

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SEC23 (300-400) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEC23A
Gene ID: 10484
Uniprot ID: SC23A_HUMAN
Immunogen Region: 300-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-400 of human Sec23AA (Q15436).
Immunogen Sequence: MVVGDELKTPIRSWHDIDKD NAKYVKKGTKHFEALANRAA TTGHVIDIYACALDQTGLLE MKCCPNLTGGYMVMGDSFNT SLFKQTFQRVFTKDMHGQFK M
Tissue Specificity Ubiquitously expressed.
Function Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex. Required for the translocation of insulin-induced glucose transporter SLC2A4/GLUT4 to the cell membrane.
Protein Name Protein Transport Protein Sec23a
Hsec23a
Sec23-Related Protein A
Database Links Reactome: R-HSA-1655829
Reactome: R-HSA-204005
Reactome: R-HSA-2132295
Reactome: R-HSA-5694530
Reactome: R-HSA-9705671
Reactome: R-HSA-983170
Cellular Localisation Cytoplasmic Vesicle
Copii-Coated Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Endoplasmic Reticulum Membrane
Cytoplasm
Cytosol
Enriched At Endoplasmic Reticulum Exit Sites
Also Known As Transitional Endoplasmic Reticulum (Ter)
Alternative Antibody Names Anti-Protein Transport Protein Sec23a antibody
Anti-Hsec23a antibody
Anti-Sec23-Related Protein A antibody
Anti-SEC23A antibody

Information sourced from Uniprot.org

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