| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-SEC13 (1-322) is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SEC13 |
| Gene ID: | 6396 |
| Uniprot ID: | SEC13_HUMAN |
| Immunogen Region: | 1-322 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-322 of human SEC13 (NP_899195.1). |
| Immunogen Sequence: | MVSVINTVDTSHEDMIHDAQ MDYYGTRLATCSSDRSVKIF DVRNGGQILIADLRGHEGPV WQVAWAHPMYGNILASCSYD RKVIIWREENGTWEKSHEHA GHDSSVNSVCWAPHDYGLIL ACGSSDGAISLLTYTGEGQW EVKKINNAHTIGCNAVSWAP AVVPGSLIDHPSGQKPNYIK RFASGGCDNLIKLWKEEEDG QWKEEQKLEAHSDWVRDVAW APSIGLPTSTIASCSQDGR |
| Function | Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. Required for the exit of adipsin (CFD/ADN), an adipocyte-secreted protein from the endoplasmic reticulum. As a component of the GATOR2 complex, functions as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway. The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex. GATOR2 probably acts as a E3 ubiquitin-protein ligase toward GATOR1. In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation. In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex. Within the GATOR2 complex, SEC13 and SEH1L are required to stabilize the complex. |
| Protein Name | Protein Sec13 HomologGator2 Complex Protein Sec13Sec13-Like Protein 1Sec13-Related Protein |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-141444Reactome: R-HSA-159227Reactome: R-HSA-159230Reactome: R-HSA-159231Reactome: R-HSA-159236Reactome: R-HSA-165054Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-168325Reactome: R-HSA-168333Reactome: R-HSA-170822Reactome: R-HSA-180746Reactome: R-HSA-180910Reactome: R-HSA-191859Reactome: R-HSA-204005Reactome: R-HSA-2132295Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-3108214Reactome: R-HSA-3232142Reactome: R-HSA-3301854Reactome: R-HSA-3371453Reactome: R-HSA-4085377Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-5578749Reactome: R-HSA-5619107Reactome: R-HSA-5663220Reactome: R-HSA-6784531Reactome: R-HSA-68877Reactome: R-HSA-9609690Reactome: R-HSA-9610379Reactome: R-HSA-9615933Reactome: R-HSA-9639288Reactome: R-HSA-9648025Reactome: R-HSA-9705671Reactome: R-HSA-983170 |
| Cellular Localisation | Cytoplasmic VesicleCopii-Coated Vesicle MembranePeripheral Membrane ProteinCytoplasmic SideEndoplasmic Reticulum MembraneNucleusNuclear Pore ComplexLysosome MembraneIn InterphaseLocalizes At Both Sides Of The Npc |
| Alternative Antibody Names | Anti-Protein Sec13 Homolog antibodyAnti-Gator2 Complex Protein Sec13 antibodyAnti-Sec13-Like Protein 1 antibodyAnti-Sec13-Related Protein antibodyAnti-SEC13 antibodyAnti-D3S1231E antibodyAnti-SEC13A antibodyAnti-SEC13L1 antibodyAnti-SEC13R antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and SPK knockout (KO) 293T cells, using [KO Validated] SPK Rabbit polyclonal antibody (STJ11100823) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89759/358235/STJ11100823_1__81500.1713122567.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SPK knockout (KO) 293T cells, using [KO Validated] SPK Rabbit polyclonal antibody (STJ11100823) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
