• Immunofluorescence analysis of U2OS cells using SEC11A Rabbit polyclonal antibody (STJ112570) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using SEC11A Rabbit polyclonal antibody (STJ112570) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SEC11A Rabbit polyclonal antibody (STJ112570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SEC11A antibody (37-145) (STJ112570)

SKU:
STJ112570

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEC11A (37-145) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEC11A
Gene ID: 23478
Uniprot ID: SC11A_HUMAN
Immunogen Region: 37-145
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-145 of human SEC11A (NP_055115.1).
Immunogen Sequence: KGLMVITGSESPIVVVLSGS MEPAFHRGDLLFLTNRVEDP IRVGEIVVFRIEGREIPIVH RVLKIHEKQNGHIKFLTKGD NNAVDDRGLYKQGQHWLEKK DVVGRARGF
Function Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.
Protein Name Signal Peptidase Complex Catalytic Subunit Sec11a
Endopeptidase Sp18
Microsomal Signal Peptidase 18 Kda Subunit
Spase 18 Kda Subunit
Sec11 Homolog A
Sec11-Like Protein 1
Spc18
Database Links Reactome: R-HSA-1799339
Reactome: R-HSA-381771
Reactome: R-HSA-400511
Reactome: R-HSA-422085
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Signal Peptidase Complex Catalytic Subunit Sec11a antibody
Anti-Endopeptidase Sp18 antibody
Anti-Microsomal Signal Peptidase 18 Kda Subunit antibody
Anti-Spase 18 Kda Subunit antibody
Anti-Sec11 Homolog A antibody
Anti-Sec11-Like Protein 1 antibody
Anti-Spc18 antibody
Anti-SEC11A antibody
Anti-SEC11L1 antibody
Anti-SPC18 antibody
Anti-SPCS4A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance