• Immunohistochemistry analysis of paraffin-embedded Human gastrointestinal stromal tumor using SDHB Rabbit polyclonal antibody (STJ11104664) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SDHB antibody (STJ11104664) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-SDHB antibody (29-280) (STJ11104664)

SKU:
STJ11104664

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Succinate Dehydrogenase Ubiquinone Iron-Sulfur Subunit-Mitochondrial (29-280) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SDHB
Gene ID: 6390
Uniprot ID: SDHB_HUMAN
Immunogen Region: 29-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-280 of human SDHB (NP_002991.2).
Immunogen Sequence: AQTAAATAPRIKKFAIYRWD PDKAGDKPHMQTYEVDLNKC GPMVLDALIKIKNEVDSTLT FRRSCREGICGSCAMNINGG NTLACTRRIDTNLNKVSKIY PLPHMYVIKDLVPDLSNFYA QYKSIEPYLKKKDESQEGKQ QYLQSIEEREKLDGLYECIL CACCSTSCPSYWWNGDKYLG PAVLMQAYRWMIDSRDDFTE ERLAKLQDPFSLYRCHTIMN CTRTCPKGLNPGKAIAEIK
Function Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
Protein Name Succinate Dehydrogenase Ubiquinone Iron-Sulfur Subunit - Mitochondrial
Iron-Sulfur Subunit Of Complex Ii
Ip
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Succinate Dehydrogenase Ubiquinone Iron-Sulfur Subunit - Mitochondrial antibody
Anti-Iron-Sulfur Subunit Of Complex Ii antibody
Anti-Ip antibody
Anti-SDHB antibody
Anti-SDH antibody
Anti-SDH1 antibody

Information sourced from Uniprot.org

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