• Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SDCCAG8 antibody (520-600) (STJ94581)

SKU:
STJ94581

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Serologically defined colon cancer antigen 8 (520-600) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SDCCAG8
Gene ID: 10806
Uniprot ID: SDCG8_HUMAN
Immunogen Region: 520-600
Specificity: NY-CO-8 Polyclonal Antibody detects endogenous levels of NY-CO-8 protein.
Immunogen: Synthesized peptide derived from NY-CO-8. at AA range: 520-600
Tissue Specificity Expressed in thymus, prostate, testis, ovary, small intestine, colon, mucosa, colon and renal cancer tumors.
Function Plays a role in the establishment of cell polarity and epithelial lumen formation. Also plays an essential role in ciliogenesis and subsequent Hedgehog signaling pathway that requires the presence of intact primary cilia for pathway activation. Mechanistically, interacts with and mediates RABEP2 centrosomal localization which is critical for ciliogenesis.
Protein Name Serologically Defined Colon Cancer Antigen 8
Antigen Ny-Co-8
Centrosomal Colon Cancer Autoantigen Protein
Hcccap
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Cilium Basal Body
Cell Junction
Located At The Distal Ends Of Both Centrioles And Colocalizes To Centrosomes Throughout The Cell Cycle
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Serologically Defined Colon Cancer Antigen 8 antibody
Anti-Antigen Ny-Co-8 antibody
Anti-Centrosomal Colon Cancer Autoantigen Protein antibody
Anti-Hcccap antibody
Anti-SDCCAG8 antibody
Anti-CCCAP antibody
Anti-NPHP10 antibody
Anti-HSPC085 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance