• Immunohistochemistry analysis of SDC2 in paraffin-embedded human esophagus using SDC2 Rabbit polyclonal antibody (STJ25458) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SDC2 in paraffin-embedded human liver damage using SDC2 Rabbit polyclonal antibody (STJ25458) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SDC2 in paraffin-embedded mouse lung using SDC2 Rabbit polyclonal antibody (STJ25458) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SDC2 Rabbit polyclonal antibody (STJ25458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SDC2 antibody (19-144) (STJ25458)

SKU:
STJ25458

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SDC2 (19-144) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SDC2
Gene ID: 6383
Uniprot ID: SDC2_HUMAN
Immunogen Region: 19-144
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-144 of human SDC2 (NP_002989.2).
Immunogen Sequence: ESRAELTSDKDMYLDNSSIE EASGVYPIDDDDYASASGSG ADEDVESPELTTSRPLPKIL LTSAAPKVETTTLNIQNKIP AQTKSPEETDKEKVHLSDSE RKMDPAEEDTNVYTEKHSDS LFKRTE
Post Translational Modifications O-glycosylated with core 1 or possibly core 8 glycans. Contains heparan sulfate. Also contains chondroitin sulfate.
Function Cell surface proteoglycan which regulates dendritic arbor morphogenesis.
Protein Name Syndecan-2
Synd2
Fibroglycan
Heparan Sulfate Proteoglycan Core Protein
Hspg
Cd Antigen Cd362
Database Links Reactome: R-HSA-1971475
Reactome: R-HSA-2022928
Reactome: R-HSA-2024096
Reactome: R-HSA-202733
Reactome: R-HSA-3000170
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Reactome: R-HSA-381426
Reactome: R-HSA-3928662
Reactome: R-HSA-4420332
Reactome: R-HSA-8957275
Reactome: R-HSA-9694614
Reactome: R-HSA-975634
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Syndecan-2 antibody
Anti-Synd2 antibody
Anti-Fibroglycan antibody
Anti-Heparan Sulfate Proteoglycan Core Protein antibody
Anti-Hspg antibody
Anti-Cd Antigen Cd362 antibody
Anti-SDC2 antibody
Anti-HSPG1 antibody

Information sourced from Uniprot.org

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