• Immunofluorescence analysis of L929 cells using Secretagogin (SCGN) Rabbit polyclonal antibody (STJ114763) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Secretagogin (SCGN) in paraffin-embedded Rat pancreas using Secretagogin (Secretagogin (SCGN) ) Rabbit polyclonal antibody (STJ114763) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Secretagogin (SCGN) in paraffin-embedded Human breast cancer using Secretagogin (Secretagogin (SCGN) ) Rabbit polyclonal antibody (STJ114763) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Secretagogin (SCGN) Rabbit polyclonal antibody (STJ114763) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SCGN antibody (1-276) (STJ114763)

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STJ114763

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SCGN (1-276) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCGN
Gene ID: 10590
Uniprot ID: SEGN_HUMAN
Immunogen Region: 1-276
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-276 of human Secretagogin (Secretagogin (SCGN) ) (NP_008929.2).
Immunogen Sequence: MDSSREPTLGRLDAAGFWQV WQRFDADEKGYIEEKELDAF FLHMLMKLGTDDTVMKANLH KVKQQFMTTQDASKDGRIRM KELAGMFLSEDENFLLLFRR ENPLDSSVEFMQIWRKYDAD SSGFISAAELRNFLRDLFLH HKKAISEAKLEEYTGTMMKI FDRNKDGRLDLNDLARILAL QENFLLQFKMDACSTEERKR DFEKIFAYYDVSKTGALEGP EVDGFVKDMMELVQPSISG
Tissue Specificity Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons.
Protein Name Secretagogin
Cellular Localisation Cytoplasm
Secreted
Cytoplasmic Vesicle
Secretory Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Predominantly Cytoplasmic
A Small Proportion Is Associated With Secretory Granules And Membrane Fractions
Detectable In Human Serum After Ischemic Neuronal Damage
Alternative Antibody Names Anti-Secretagogin antibody
Anti-SCGN antibody
Anti-SECRET antibody

Information sourced from Uniprot.org

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