• Western blot analysis of extracts of HeLa cells, using SCAF8 antibody (STJ11103029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using SCAF8 antibody (STJ11103029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SCAF8 antibody (572-634) (STJ11103029)

SKU:
STJ11103029

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SCAF8 (572-634) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCAF8
Gene ID: 22828
Uniprot ID: SCAF8_HUMAN
Immunogen Region: 572-634
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 572-634 of human SCAF8 (NP_055707.3).
Immunogen Sequence: PWEKVKVDDLEGFAEGGMID QETVNTEWETVKSSEPVKET VQTTQSPTPVEKETVVTTQA EVF
Function Anti-terminator protein required to prevent early mRNA termination during transcription. Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins. Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation. Independently of SCAF4, also acts as a positive regulator of transcript elongation.
Protein Name Sr-Related And Ctd-Associated Factor 8
Cdc5l Complex-Associated Protein 7
Rna-Binding Motif Protein 16
Cellular Localisation Nucleus
Nucleus Matrix
Detected In Granular Nuclear Foci Which Correspond To Sites Of Active Transcription
Alternative Antibody Names Anti-Sr-Related And Ctd-Associated Factor 8 antibody
Anti-Cdc5l Complex-Associated Protein 7 antibody
Anti-Rna-Binding Motif Protein 16 antibody
Anti-SCAF8 antibody
Anti-CCAP7 antibody
Anti-KIAA1116 antibody
Anti-RBM16 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance