Anti-SCAF8 antibody (572-634) (STJ11103029)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SCAF8 (572-634) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SCAF8 |
| Gene ID | 22828 |
| Uniprot ID | SCAF8_HUMAN |
| Immunogen Region | 572-634 |
| Immunogen Sequence | PWEKVKVDDLEGFAEGGMID QETVNTEWETVKSSEPVKET VQTTQSPTPVEKETVVTTQA EVF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 572-634 of human SCAF8 (NP_055707.3). |
Additional Info
| Function | Anti-terminator protein required to prevent early mRNA termination during transcription. Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins. Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation. Independently of SCAF4, also acts as a positive regulator of transcript elongation. |
| Protein Name | Sr-Related And Ctd-Associated Factor 8Cdc5l Complex-Associated Protein 7Rna-Binding Motif Protein 16 |
| Database Links | |
| Cellular Localisation | NucleusNucleus MatrixDetected In Granular Nuclear Foci Which Correspond To Sites Of Active Transcription |
| Alternative Antibody Names | Anti-Sr-Related And Ctd-Associated Factor 8 antibodyAnti-Cdc5l Complex-Associated Protein 7 antibodyAnti-Rna-Binding Motif Protein 16 antibodyAnti-SCAF8 antibodyAnti-CCAP7 antibodyAnti-KIAA1116 antibodyAnti-RBM16 antibody |
Information sourced from Uniprot.org