• Western blot analysis of extracts of various cell lines, using SART1 antibody (STJ111304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunoprecipitation analysis of 150 Mu g extracts of Jurkat cells using 3 Mu g SART1 antibody (STJ111304). Western blot was performed from the immunoprecipitate using SART1 antibody (STJ111304) at a dilution of 1:1000.
  • Immunohistochemistry of paraffin-embedded human esophageal using SART1 rabbit polyclonal antibody (STJ111304) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of SART1 in paraffin-embedded rat brain using SART1 Rabbit polyclonal antibody (STJ111304) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using SART1 rabbit polyclonal antibody (STJ111304) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of SART1 in paraffin-embedded mouse kidney using SART1 Rabbit polyclonal antibody (STJ111304) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using SART1 rabbit polyclonal antibody (STJ111304) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of SART1 in paraffin-embedded human esophageal using SART1 Rabbit polyclonal antibody (STJ111304) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SART1 antibody (STJ111304). Western blot was performed from the immunoprecipitate using SART1 antibody (STJ111304) at a dilition of 1:1000.
  • Western blot analysis of various lysates using SART1 Rabbit polyclonal antibody (STJ111304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SART1 antibody (1-255) (STJ111304)

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STJ111304

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SART1 (1-255) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SART1
Gene ID: 9092
Uniprot ID: SNUT1_HUMAN
Immunogen Region: 1-255
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human SART1 (NP_005137.1).
Immunogen Sequence: MGSSKKHRGEKEAAGTTAAA GTGGATEQPPRHREHKKHKH RSGGSGGSGGERRKRSRERG GERGSGRRGAEAEARSSTHG RERSQAEPSERRVKREKRDD GYEAAASSKTSSGDASSLSI EETNKLRAKLGLKPLEVNAI KKEAGTKEEPVTADVINPMA LRQREELREKLAAAKEKRLL NQKLGKIKTLGEDDPWLDDT AAWIERSRQLQKEKDLAEKR AKLLEEMDQEFGVSTLVEE
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Sumoylated with SUMO2.
Function Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.
Protein Name U4/U6.U5 Tri-Snrnp-Associated Protein 1
Snu66 Homolog
Hsnu66
Squamous Cell Carcinoma Antigen Recognized By T-Cells 1
Sart-1
Hsart-1
U4/U6.U5 Tri-Snrnp-Associated 110 Kda Protein
Allergen Hom S 1
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Found In The Nucleus Of Mitogen-Activated Peripheral Blood Mononuclear Cells (Pbmcs)
Tumor Cells
Or Normal Cell Lines
But Not In Normal Tissues Except Testis And Fetal Liver Or In Unstimulated Pbmcs
Suggesting Preferential Expression In Proliferating Cells
Alternative Antibody Names Anti-U4/U6.U5 Tri-Snrnp-Associated Protein 1 antibody
Anti-Snu66 Homolog antibody
Anti-Hsnu66 antibody
Anti-Squamous Cell Carcinoma Antigen Recognized By T-Cells 1 antibody
Anti-Sart-1 antibody
Anti-Hsart-1 antibody
Anti-U4/U6.U5 Tri-Snrnp-Associated 110 Kda Protein antibody
Anti-Allergen Hom S 1 antibody
Anti-SART1 antibody

Information sourced from Uniprot.org

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