Anti-SARS-CoV-2-Spike S2 ECD antibody (686-1214) (STJ11103173)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SARS-CoV-2-Spike S2 ECD (686-1214) antibody for use in WB, DB, IF, ICC, IP and ELISA in human and sars-cov-2 samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/DB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/SARS-CoV-2 |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:2000-1:6000DB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Immunogen Region | 686-1214 |
| Immunogen Sequence | SVASQSIIAYTMSLGAENSV AYSNNSIAIPTNFTISVTTE ILPVSMTKTSVDCTMYICGD STECSNLLLQYGSFCTQLNR ALTGIAVEQDKNTQEVFAQV KQIYKTPPIKDFGGFNFSQI LPDPSKPSKRSFIEDLLFNK VTLADAGFIKQYGDCLGDIA ARDLICAQKFNGLTVLPPLL TDEMIAQYTSALLAGTITSG WTFGAGAALQIPFAMQMAYR FNGIGVTQNVLYENQKLIA |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 686-1214 of coronavirus Spike S2 ECD (YP_009724390.1). |
Additional Info
| Background | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E) , spike or surface glycoprotein (S) , membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests. |
Information sourced from Uniprot.org