• Immunoprecipitation analysis of 600 Mu g extracts of Mouse heart cells using 3 Mu g SAMM50 antibody (STJ25440). Western blot was performed from the immunoprecipitate using SAMM50 antibody (STJ25440) at a dilution of 1:1000.
  • Immunohistochemistry analysis of SAMM50 in paraffin-embedded mouse heart using SAMM50 Rabbit polyclonal antibody (STJ25440) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SAMM50 in paraffin-embedded human liver cancer using SAMM50 Rabbit polyclonal antibody (STJ25440) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SAMM50 in paraffin-embedded rat heart using SAMM50 Rabbit polyclonal antibody (STJ25440) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SAMM50 Rabbit polyclonal antibody (STJ25440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SAMM50 antibody (1-220) (STJ25440)

SKU:
STJ25440

$151.88 - $708.08
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Host: Rabbit
Applications: WB/IHC-P/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SAMM50
Gene ID: 25813
Uniprot ID: SAM50_HUMAN
Immunogen Region: 1-220
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SAMM50 (NP_056195.3).
Immunogen Sequence: MGTVHARSLEPLPSSGPDFG GLGEEAEFVEVEPEAKQEIL ENKDVVVQHVHFDGLGRTKD DIIICEIGDVFKAKNLIEVM RKSHEAREKLLRLGIFRQVD VLIDTCQGDDALPNGLDVTF EVTELRRLTGSYNTMVGNNE GSMVLGLKLPNLLGRAEKVT FQFSYGTKETSYGLSFFKPR PGNFERNFSVNLYKVTGQFP WSSLRETDRGMSAEYSFPIW
Function Plays a crucial role in the maintenance of the structure of mitochondrial cristae and the proper assembly of the mitochondrial respiratory chain complexes. Required for the assembly of TOMM40 into the TOM complex.
Protein Name Sorting And Assembly Machinery Component 50 Homolog
Transformation-Related Gene 3 Protein
Trg-3
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-8949613
Reactome: R-HSA-9013404
Cellular Localisation Mitochondrion Outer Membrane
Multi-Pass Membrane Protein
Cytoplasm
Mitochondrion
Alternative Antibody Names Anti-Sorting And Assembly Machinery Component 50 Homolog antibody
Anti-Transformation-Related Gene 3 Protein antibody
Anti-Trg-3 antibody
Anti-SAMM50 antibody
Anti-SAM50 antibody
Anti-CGI-51 antibody
Anti-TRG3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance