• Western blot analysis of extracts of various cell lines, using SAFB2 antibody (STJ114424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human placenta using SAFB2 rabbit polyclonal antibody (STJ114424) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse brain using SAFB2 rabbit polyclonal antibody (STJ114424) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse kidney using SAFB2 rabbit polyclonal antibody (STJ114424) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat testis using SAFB2 rabbit polyclonal antibody (STJ114424) at dilution of 1:100 (40x lens).

Anti-SAFB2 antibody (1-255) (STJ114424)

SKU:
STJ114424

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SAFB2 (1-255) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SAFB2
Gene ID: 9667
Uniprot ID: SAFB2_HUMAN
Immunogen Region: 1-255
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human SAFB2 (NP_055464.1).
Immunogen Sequence: MAETLPGSGDSGPGTASLGP GVAETGTRRLSELRVIDLRA ELKKRNLDTGGNKSVLMERL KKAVKEEGQDPDEIGIELEA TSKKSAKRCVKGLKMEEEGT EDNGLEDDSRDGQEDMEASL ENLQNMGMMDMSVLDETEVA NSSAPDFGEDGTDGLLDSFC DSKEYVAAQLRQLPAQPPEH AVDGEGFKNTLETSSLNFKV TPDIEESLLEPENEKILDIL GETCKSEPVKEESSELEQP
Tissue Specificity Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
Function Binds to scaffold/matrix attachment region (S/MAR) DNA. Can function as an estrogen receptor corepressor and can also inhibit cell proliferation.
Protein Name Scaffold Attachment Factor B2
Saf-B2
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Scaffold Attachment Factor B2 antibody
Anti-Saf-B2 antibody
Anti-SAFB2 antibody
Anti-KIAA0138 antibody

Information sourced from Uniprot.org

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